Protective effects of lycopene against ultraviolet B-induced photodamage
Protective effects of lycopene against ultraviolet B-induced photodamage
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DOI：
10.1207/s15327914nc4702_11
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发表时间：
2003-01-01
影响因子：
2.9
通讯作者：
Wei, HC
中科院分区：
文献类型：
--
作者：
Fazekas, Z;Gao, DY;Wei, HC
番茄红素是番茄及其产品中发现的一种无环烃类胡萝卜素，是一种公认的有效抗氧化剂，其抗癌特性已在培养细胞和动物模型中得到证明。我们研究了两种浓度的外用番茄红素对急性紫外线 B (UVB) 引起的光损伤的保护作用。番茄红素的应用剂量依赖性地抑制UVB诱导的鸟氨酸脱羧酶（P < 0.05）和髓过氧化物酶（P < 0.05），并显着减少双褶皮肤厚度（P < 0.05）。免疫组织化学染色显示，与未暴露的对照组相比，UVB 暴露组中凋亡途径的活性 caspase-3 增加。局部使用番茄红素可防止 caspase-3 的裂解。 UVB 照射完全减少了增殖细胞核抗原 (PCNA)，并且未经处理的皮肤在整个基底表皮中保持了阳性染色的细胞。局部应用番茄红素可显着逆转 UVB 诱导的 PCNA 抑制，并且经过番茄红素处理的皮肤恢复正常的 PCNA 染色。我们的结果表明，外用番茄红素能够发挥其针对急性 UVB 引起的光损伤的保护作用。此外，它还可以通过抑制表皮鸟氨酸脱羧酶活性、减少炎症反应、维持正常细胞增殖以及可能通过阻断 UVB 损伤后细胞凋亡的必要步骤来预防 DNA 损伤，从而起到预防剂的作用。
Lycopene, an acyclic hydrocarbon carotenoid found in tomatoes and their products, is a well-established potent antioxidant, and its anticancer properties have been shown in cultured cells and animal models. We investigated the protective effects of two concentrations of topical lycopene against acute ultraviolet B (UVB)-induced photo-damage. Application of lycopene dose dependently inhibited UVB-induced ornithine decarboxylase (P < 0.05) and myeloperoxidase (P < 0.05) and significantly reduced bifold skin thickness (P < 0.05). Immunohistochemical staining revealed increased active caspase-3 of apoptotic pathway in the UVB-exposed group compared with the unexposed control. Application of topical lycopene prevented the cleavage of caspase-3. UVB irradiation completely diminished proliferating cell nuclear antigen (PCNA), and the untreated skin maintained positively stained cells throughout the basal epidermis. Topical application of lycopene significantly reversed UVB-induced PCNA inhibition, and normal PCNA staining was restored in the lycopene-treated skin. Our results suggest that topical lycopene is able to exert its protective effects against acute UVB-induced photodamage. Furthermore, it may act as a preventative agent via inhibition of epidermal ornithine decarboxylase activity, reducing inflammatory responses, maintaining normal cell proliferation, and possibly preventing DNA damage as indicated by blocking the necessitating step of apoptosis following UVB injury.