WALKING ALONG THE RABIES GENOME - IS THE LARGE G-L INTERGENIC REGION A REMNANT GENE
WALKING ALONG THE RABIES GENOME - IS THE LARGE G-L INTERGENIC REGION A REMNANT GENE
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DOI：
10.1073/pnas.83.11.3914
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发表时间：
1986-06-01
影响因子：
11.1
通讯作者：
ROUGEON, F
中科院分区：
文献类型：
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作者：
TORDO, N;POCH, O;ROUGEON, F
通过使用两个连续的DNA引物“沿基因组沿着行走”获得的狂犬病cDNA克隆，已经允许对编码N、M1、M2、G和L蛋白的开始以及狂犬病基因间区域的基因进行序列测定。位于编码蛋白质的每个基因的边界处的起始和终止转录信号已经被鉴定，并且与来自水泡性口炎病毒（VSV）和仙台病毒的相应信号相似。除了核蛋白的有限延伸外，这三种病毒的相应结构蛋白之间没有同源性。狂犬病基因间区在长度和序列上都是可变的。在423个核苷酸长的G-L基因间区存在一个残基蛋白基因的证据。这一发现是讨论的不分段负链RNA病毒的进化。
Rabies cDNA clones, obtained by "walking along the genome" using two successive DNA primers, have allowed the sequence determination of the genes encoding the N, M1, M2, G, and the beginning of the L protein as well as the rabies intergenic regions. Start and stop transcription signals located at the border of each gene encoding a protein have been identified and are similar to the corresponding signals from vesicular stomatitis virus (VSV) and Sendai virus. Except for limited stretches of the nucleoprotein, there is no homology between corresponding structural proteins of these three viruses. Rabies intergenic regions are variable both in length and sequence. Evidence for the existence of a remnant protein gene in the 423 nucleotide long G-L intergenic region is presented. This finding is discussed in terms of the evolution of unsegmented negative-strand RNA viruses.