Immunoassay for quantifying type I collagen degradation products in urine evaluated.
Immunoassay for quantifying type I collagen degradation products in urine evaluated.
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评估尿液中 I 型胶原降解产物的免疫测定。
DOI：
10.1093/clinchem/40.11.2022
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发表时间：
1994
影响因子：
9.3
通讯作者：
C. Christiansen
中科院分区：
文献类型：
--
作者：
M. Bonde;P. Qvist;C. Fledelius;B. Riis;C. Christiansen
建立检测尿中I型胶原降解产物的酶联免疫吸附试验(EL ISA)。方法的检测范围为0.5~10.5 mg/L，检出限为0.2 mg/L，批内变异系数为5.3%，总变异系数为6.6%。分析回收率平均为100%。绝经前健康人群(n=102)的尿样(+/-SD)平均浓度为250+/-110 mg/摩尔肌酐(Cr)。一组健康绝经后妇女(n=410)的血肌酸值为416+/-189 mg/molCr。与已建立的骨吸收标记物脱氧吡啶(n=214)的HPLC值有很好的相关性(r=0.83)，略好于与羟脯氨酸的相关性(r=0.78，n=421)。我们的结论是，这里描述的检测方法为进一步阐明尿中I型胶原降解产物的重要性提供了一个有用的工具。
An enzyme-linked immunosorbent assay (ELISA) for measuring type I collagen degradation products in urine < 3 h was evaluated. The measuring range was 0.5-10.5 mg/L with a detection limit of 0.2 mg/L. Within-run and total CVs were 5.3% and 6.6%, respectively. Analytical recovery averaged 100%. The mean (+/- SD) concentrations in urine samples from a healthy premenopausal population (n = 102) were 250 +/- 110 mg/mol creatinine (Cr). A group of healthy postmenopausal women (n = 410) gave a mean value of 416 +/- 189 mg/mol Cr. Values obtained in the ELISA correlated well (r = 0.83) to HPLC values for the established bone resorption marker deoxypyridinoline (n = 214), slightly better than the correlation to hydroxyproline measurements (r = 0.78, n = 421). We conclude that the assay described here presents a useful tool for further elucidating the importance of type I collagen degradation products in urine.
影响因子：
2.9
作者：
EYRE, DR;KOOB, TJ;VANNESS, KP
通讯作者：
VANNESS, KP