TRANSCRIPTION INITIATION OF THE SACCHAROMYCES-CEREVISIAE ISO-1-CYTOCHROME-C GENE MULTIPLE, INDEPENDENT T-A-T-A SEQUENCES
TRANSCRIPTION INITIATION OF THE SACCHAROMYCES-CEREVISIAE ISO-1-CYTOCHROME-C GENE MULTIPLE, INDEPENDENT T-A-T-A SEQUENCES
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DOI：
10.1016/0022-2836(86)90439-0
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发表时间：
1986-02-05
影响因子：
5.6
通讯作者：
SMITH, M
中科院分区：
文献类型：
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作者：
MCNEIL, JB;SMITH, M
酿酒酵母Cyc1基因编码iso-1-细胞色素c，它的表达产生了一个信使RNA家族，其5‘末端位于蛋白质编码DNA序列+1位的第一个核苷酸的+7-93位区域。用链接子扫描缺失和基因融合的方法研究了Cyc1基因转录启动的机制。构建了在5‘非编码区有突变的不同的Cyc1衍生物，利用多拷贝质粒将其重新导入酵母，并通过引物延伸定位了mRNA的起始位置。结果表明，4个或可能5个T-A-T-A序列位于Cyc1基因的5‘非编码区，每个T-A-T-A序列都是特定的mRNA启动子集所必需的。这一结论已被染色体上的Cyc1T-A-T-A序列的寡核苷酸突变所证实。T-A-T-A序列和mRNA起始点之间也存在松散的空间关系，这种距离关系从100到60个碱基对(.+-.15个碱基对)。
The expression of the Saccharomyces cerevisiae CYC1 gene, which encodes iso-1-cytochrome c, produces a family of messenger RNAs whose 5'' ends map in the region from position +7 to -93 relative to the first nucleotide at position +1 of the protein-coding DNA sequence. The mechanism of transcription initiation of the CYC1 gene has been examined by using linker-scanning deletions and gene fusions. The various CYC1 derivatives with mutations in the 5'' non-coding region were constructed, reintroduced into yeast using a multicopy plasmid, and the mRNA starts mapped by primer extension. The results indicate that four, and possibly five T-A-T-A sequences are located within the 5'' non-coding region of the CYC1 gene, and that each T-A-T-A is required for a specific subset of mRNA starts. This conclusion has been confirmed by oligonucleotide mutagenesis of a chromosomal CYC1 T-A-T-A sequence. A loose spatial relationship also exists between the T-A-T-A sequences and the mRNA start sites, and this distance relationship varies from 100 to 60 base-pairs (.+-. 15 base-pairs).