HORSERADISH-PEROXIDASE LABELING OF THE EFFERENT AND AFFERENT PATHWAYS OF THE AVIAN TANGENTIAL VESTIBULAR NUCLEUS
HORSERADISH-PEROXIDASE LABELING OF THE EFFERENT AND AFFERENT PATHWAYS OF THE AVIAN TANGENTIAL VESTIBULAR NUCLEUS
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DOI：
10.1002/cne.902960211
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发表时间：
1990-06-08
影响因子：
2.5
通讯作者：
PEUSNER, KD
中科院分区：
文献类型：
--
作者：
COX, RG;PEUSNER, KD
用辣根过氧化物酶(HRP：Sigma VI型)标记神经胞体和纤维，研究了雏鸡切线核的传出和传入通路。HRP进入切线核以及进入脊髓第二颈椎水平的研究表明，离开切线核时，切线神经元的轴突形成一个向前走行的束，穿过前庭腹外侧核，没有分支，然后到达对侧内侧纵束(MLF)。在MLF内，切向轴突向后移动，形成支配外展神经核的侧支，然后进入颈髓。在1天、1周和7周龄的动物中，两个主要神经元的轴突--主细胞和细长细胞--都证明了这一途径。此外，我们提出，由于HRP注射到脊髓一侧会导致切线神经元的双侧标记，因此存在一条未被识别的同侧切线轴突到达脊髓的通路。HRP定位于悬垂核、小叶核、桥脑网状结构、梨状核、巨核、前庭小脑核、背侧核或前庭内侧核的背内侧部后，切核内未见标记细胞。切核接受来自巨大的前庭纤维(勺状终末)、细小的前庭壶腹纤维侧支、小叶和高颈段脊髓的传入。从我们的小样本中可以看出，在1日龄、1周龄和7周龄的动物中，脊髓纤维形成了切向核中的大部分传入终末。
The efferent and afferent pathways of the chick tangential nucleus were studied by using horseradish peroxidase (HRP: Sigma type VI) to label nerve cell bodies and fibers. Depositions of HRP into the tangential nucleus, as well as into the second cervical level of the spinal cord, show that the axons of tangential neurons on leaving the nucleus form an anteriorly coursing tract that passes through the ventrolateral vestibular nucleus without branching and then to the contralateral medial longitudinal fasciculus (MLF). Within the MLF, the tangential axons course posteriorly, forming collaterals that innervate the abducens nucleus, and then proceed to the cervical spinal cord. This pathway was demonstrated for the axons of the two main neurons, the principal and elongate cells, in 1-day, 1-week, and 7-week-old animals. In addition, we propose the existence of an unidentified, ipsilateral pathway to the spinal cord for the tangential axons, since HRP injections into one side of the spinal cord resulted in the bilateral labeling of tangential neurons. No labeled cells were found in the tangential nucleus following HRP depositions into the uvula, flocculus, pontine reticular formation, nucleus piriformis, nucleus jumeaux, vestibulocerebellar nucleus, retrotangential nucleus, or the dorsomedial part of the medial vestibular nucleus. The tangetial nucleus receives afferents from the colossal vestibular fibers (spoon endings), small collaterals of fine vestibular ampullary fibers, flocculus, and high cervical levels of the spinal cord. From our small sample, it appears that the spinal cord fibers form most of the afferent terminals in the tangential nucleus in 1-day, 1-week, and 7-week-old animals.