Differential protein expression of kidney tissue in the scallop Patinopecten yessoensis under acute cadmium stress
Differential protein expression of kidney tissue in the scallop Patinopecten yessoensis under acute cadmium stress
复制标题
急性镉胁迫下虾夷扇贝肾组织蛋白差异表达
DOI：
10.1016/j.ecoenv.2011.02.005
复制
发表时间：
2011-07-01
影响因子：
6.8
通讯作者：
Huang, He-Qing
中科院分区：
文献类型：
--
作者：
Huang, Xiang;Fang, Cai-Wang;Huang, He-Qing
对急性氯化镉（CdCl 2）暴露的扇贝肾脏进行了形态学和蛋白质组学的观察和分析，并与未暴露的对照组进行了比较。在显微镜下，肾脏的石蜡包埋切片显示，镉暴露后，组织的微观结构已严重变形。双向电泳、MALDI-TOF质谱和数据库检索共发现13个差异表达蛋白点，其中11个表达上调，2个表达下调。其中鸟苷酸激酶（GK）和C2 H2型锌指蛋白被认为与镉的毒性密切相关。进一步采用定量PCR的方法验证了镉胁迫对GK mRNA表达的诱导作用。其他蛋白质鉴定有一定的相关性镉毒性也进行了讨论。我们认为，差异蛋白，如GK可能发挥潜在的作用，作为新的生物标志物监测镉污染的海水中的水平。(C)2011 Elsevier Inc. All rights reserved.
Morphological and proteomic changes in the kidney of scallops exposed to acute cadmium chloride (CdCl2) were observed, analyzed and compared with those in the non-exposed control group. Under microscopy the paraffin-embedded sections of the kidney revealed that the microstructure of the tissue had been severely deformed after Cd exposure. Two dimensional electrophoresis, MALDI-TOF mass spectrometry and database searches showed 13 differentially expressed protein spots, of which 11 were up-regulated, while two were down-regulated. Among these proteins, guanylate kinase (GK) and C2H2-type zinc finger protein are considered to be tightly connected with Cd toxicity. Further studies using quantitative PCR method validated that the GK mRNA was induced under Cd stress. Other proteins identified which had some relevance to Cd toxicity are also discussed. We suggested that differential proteins such as GK could play a potential role as novel biomarkers for monitoring the level of Cd contamination in seawater. (C) 2011 Elsevier Inc. All rights reserved.