Knockout of OsNramp5 using the CRISPR/Cas9 system produces low Cd-accumulating indica rice without compromising yield.
Knockout of OsNramp5 using the CRISPR/Cas9 system produces low Cd-accumulating indica rice without compromising yield.
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DOI：
10.1038/s41598-017-14832-9
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发表时间：
2017-10-31
影响因子：
4.6
通讯作者：
Zhao B
中科院分区：
文献类型：
--
作者：
Tang L;Mao B;Li Y;Lv Q;Zhang L;Chen C;He H;Wang W;Zeng X;Shao Y;Pan Y;Hu Y;Peng Y;Fu X;Li H;Xia S;Zhao B
镉（Cd）超标的米粒是膳食镉摄入的主要来源，严重威胁以大米为主食的人们的健康。开发持续低镉含量的优良水稻品种对传统育种方法来说是一个挑战，迫切需要制定新的策略。在此，我们报告了通过使用 CRISPR/Cas9 系统敲除金属转运蛋白基因 OsNramp5 开发出低镉积累且无转基因的新籼稻品系。水培培养表明，osnramp5 突变体的芽和根中的镉浓度显着降低，从而挽救了高镉条件下受损的生长。镉污染稻田试验表明，osnramp5 籽粒中的镉浓度始终低于 0.05mg/kg，而华占（野生型籼稻）籽粒中的镉浓度高达 0.33mg/kg 至 2.90mg/kg。特别是，osnramp5 突变体的植物产量没有受到显着影响。此外，我们还开发了有前景的杂交水稻品种，其谷物中的镉含量极低。我们的工作为开发镉污染安全的籼稻品种提供了一种实用的方法，最大限度地减少谷物中的镉污染风险。
Rice grain with excessive cadmium (Cd) is a major source of dietary Cd intake and a serious threat to health for people who consume rice as a staple food. The development of elite rice cultivars with consistently low Cd content is challenging for conventional breeding approaches, and new strategies urgently need to be developed. Here, we report the development of new indica rice lines with low Cd accumulation and no transgenes by knocking out the metal transporter gene OsNramp5 using CRISPR/Cas9 system. Hydroponic culture showed that Cd concentrations in shoots and roots of osnramp5 mutants were dramatically decreased, resulting in rescue of impaired growth in high Cd condition. Cd-contaminated paddy field trials demonstrated that Cd concentration in osnramp5 grains was consistently less than 0.05 mg/kg, in contrast to high Cd concentrations from 0.33 mg/kg to 2.90 mg/kg in grains of Huazhan (the wild-type indica rice). In particular, the plant yield was not significantly affected in osnramp5 mutants. Furthermore, we developed promising hybrid rice lines with extremely low Cd content in grains. Our work supplies a practical approach to developing Cd pollution-safe indica rice cultivars that minimizes Cd contamination risk in grains.
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影响因子：
2
作者：
Nakanishi, Hiromi;Ogawa, Ippei;Nishizawa, Naoko K.
通讯作者：
Nishizawa, Naoko K.
影响因子：
27.5
作者：
Ma, Xingliang;Zhang, Qunyu;Liu, Yao-Guang
通讯作者：
Liu, Yao-Guang
影响因子：
5.8
作者：
Larkin, M. A.;Blackshields, G.;Higgins, D. G.
通讯作者：
Higgins, D. G.
影响因子：
4.3
作者：
Hyun Y;Kim J;Cho SW;Choi Y;Kim JS;Coupland G
通讯作者：
Coupland G
影响因子：
4.6
作者：
Ishimaru, Yasuhiro;Takahashi, Ryuichi;Bashir, Khurram;Shimo, Hugo;Senoura, Takeshi;Sugimoto, Kazuhiko;Ono, Kazuko;Yano, Masahiro;Ishikawa, Satoru;Arao, Tomohito;Nakanishi, Hiromi;Nishizawa, Naoko K.
通讯作者：
Nishizawa, Naoko K.