Remineralising Dentine Caries Using Sodium Fluoride with Silver Nanoparticles: An In Vitro Study
Remineralising Dentine Caries Using Sodium Fluoride with Silver Nanoparticles: An In Vitro Study
复制标题
使用氟化钠和银纳米粒子再矿化牙本质龋齿：一项体外研究
DOI：
10.2147/ijn.s247550
复制
发表时间：
2020-01-01
影响因子：
8
通讯作者：
Chu, Chun Hung
中科院分区：
文献类型：
--
作者：
Zhao, Irene Shuping;Yin, Iris Xiaoxue;Chu, Chun Hung
目的：研究含聚乙二醇包覆银纳米粒子（PEG - AgNPs）的氟化钠（NaF）溶液对人工牙本质龋的再矿化及染色效果。
材料与方法：将脱矿的人牙本质块分为三组。第1组牙本质块局部应用12%的氟化氨银（SDF，氟含量14150 ppm）溶液。第2组牙本质块局部应用含PEG - AgNPs（银含量400 ppm）的2.5% NaF（氟含量11310 ppm）溶液。第3组牙本质块应用去离子水。所有牙本质块均进行为期8天的pH循环处理。使用扫描电子显微镜（SEM）观察表面形态和横截面特征。分别采用数字分光光度法、X射线衍射（XRD）、显微计算机断层扫描以及用羟脯氨酸测定法的分光光度法，评估牙本质块的颜色参数、晶体特征、病损深度和胶原蛋白降解情况。
结果：扫描电镜显示，第3组牙本质胶原蛋白暴露，而第1组和第2组未暴露。第1组至第3组的平均病损深度分别为118±7μm、121±14μm和339±20μm（第1、2组…… （此处英文似乎不完整）
ObjectiveTo investigate the remineralizing and staining effects of sodium fluoride (NaF) solution with polyethylene glycol-coated silver nanoparticles (PEG-AgNPs) on artificial dentine caries.Materials and MethodsDemineralized human dentine blocks were allocated to three groups. The blocks in group 1 underwent a topical application of a 12% silver diamine fluoride (SDF, 14,150 ppm fluoride) solution. The blocks in group 2 received a topical application of a 2.5% NaF (11,310 ppm fluoride) with PEG-AgNPs (400 ppm silver). The blocks in group 3 received deionized water. All blocks were subjected to pH cycling for 8 days. The surface morphology and cross-sectional features were investigated using scanning electron microscopy (SEM). The color parameters, crystal characteristics, lesion depth, and collagen degradation of the blocks were assessed using digital spectrophotometry, X-ray diffraction (XRD), micro-computed tomography, and spectrophotometry with a hydroxyproline assay, respectively.ResultsThe SEM showed that dentine collagen was exposed in group 3 but not in groups 1 and 2. The mean lesion depths in groups 1 to 3 were 118±7 µm, 121±14 µm, and 339±20 µm, respectively (groups1,2<3; p<0.001). The data indicated that fluoridated PEG-AgNPs introduced no significant color effect on dentine, but SDF caused distinct discoloration. The XRD indicated that silver chloride was formed in group 1, and fluorapatite was detected in groups 1 and 2. The concentration of hydroxyproline liberated from collagen was significantly less in groups 1 and 2 than in group 3.ConclusionThe use of NaF solution with PEG-AgNPs can remineralize artificial dentine caries and inhibit collagen degradation without causing significant tooth staining.