Importance of acid or alkali concentration on the removal of xylan and lignin for enzymatic cellulose hydrolysis
Importance of acid or alkali concentration on the removal of xylan and lignin for enzymatic cellulose hydrolysis
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DOI：
10.1016/j.indcrop.2014.10.031
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发表时间：
2015-02-01
影响因子：
5.9
通讯作者：
Kabel, Mirjam
中科院分区：
文献类型：
--
作者：
Martinez, Patricia Murciano;Bakker, Rob;Kabel, Mirjam
H2SO4和NaOH催化预处理对半纤维素和木质素的增溶效果与随后酶解纤维素的程度相关。研究了棕榈空果束、甘蔗甘蔗渣和大麦秸秆3种不同的草类原料。催化后的可溶性组分进行单糖和寡糖含量表征，残基进行单糖组成分析。碱预处理可广泛去除木质素。这种去除导致酶将葡聚糖转化为葡萄糖的转化率高达90% （w/w）。但是，碱性条件也引起高达50%的不必要的木聚糖损失。酸预处理导致木聚糖的增溶（70-80% (w/w)）几乎没有损失，而木质素保留。虽然适度的木聚糖增溶增加了酶促纤维素对残余葡聚糖的水解，但大量去除木聚糖却降低了它。因此，在不同的处理条件下，碱处理对木糖和葡萄糖的酶解效果最好。(C) 2014 Elsevier B.V.版权所有
The effect of hemicellulose and lignin solubilisation by H2SO4 and NaOH catalysed pretreatments was correlated to the extent of subsequent enzymatic cellulose hydrolysis. Three different grass-type feedstocks, palm empty fruit bunch, sugarcane bagasse and barley straw, were investigated. Soluble fractions after catalysis were characterised for mono- and oligosaccharides contents, while the residues were analysed for constituent monosaccharides composition.Alkali pretreatment resulted into extensive lignin removal. This removal resulted in up to 90% (w/w) conversion of glucan into glucose by enzymes. But, the alkaline conditions also provoked up to 50% unwanted xylan losses. Acid pretreatment resulted into solubilisation (70-80% (w/w)) of xylan with almost no losses, while lignin remained. Although moderate xylan solubilisation increased enzymatic cellulose hydrolysis of residual glucan, extensive removal of xylan decreased it. Therefore, under the treatment conditions, the alkali treatments were the most efficient in terms of enzymatic release of xylose and glucose from the insoluble residues. (C) 2014 Elsevier B.V. All rights reserved.