Primary and Metastatic Pancreatic Cancer Cells Exhibit Differential Migratory Potentials.
Primary and Metastatic Pancreatic Cancer Cells Exhibit Differential Migratory Potentials.
复制标题
原发性和转移性胰腺癌细胞表现出差异的迁移潜力。
DOI：
10.1097/mpa.0000000000001459
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发表时间：
2020
期刊：
影响因子：
2.9
通讯作者：
Liss,AndrewS
中科院分区：
文献类型：
--
作者：
Park,JooKyung;Hank,Thomas;Scherber,CallyM;Lillemoe,KeithD;Fernández-DelCastillo,Carlos;Warshaw,AndrewL;Toner,Mehmet;Irimia,Daniel;Thayer,SarahP;Liss,AndrewS
ObjectivesPancreatic ductal adenocarcinoma (PDAC) is characterized by early metastatic spread in more than 50% of patients. In this study, we sought to understand the migratory properties of (non) metastatic PDAC cells and determine whether the migration of cancer stem cell (CSC) populations accounts for the aggressive nature of this disease.MethodsThe migratory abilities of primary and metastatic PDAC cell lines were investigated using a microfluidic device and time-lapse photography. The velocity, time of delay of mobilization, and number of migratory cells were analyzed. Cancer stem cell subpopulations were isolated by fluorescence-activated cell sorting and their migratory properties compared with their non-CSC counterparts.ResultsPrimary cancer cells exhibited higher velocities, greater number of migratory cells, and a shorter time of delay of mobilization in comparison to metastatic cell lines. Characterization of CSC populations revealed primary PDAC cell lines were composed of fewer CD133+ and CD24+ CD44+ CSC subpopulations than metastatic cells. Moreover, migratory analysis of CSC subpopulations revealed lower velocities, fewer migratory cells, and a greater time of delay of mobilization than non-CSC.ConclusionsPrimary cancer cells demonstrate enhanced migratory abilities in comparison to metastatic PDAC cells. Those differences may result from lower CSC subpopulations in primary cells because CSC populations demonstrated impaired migratory abilities in contrast to non-CSC.
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癌症干细胞生态位：在调节肿瘤细胞的干性中，小众的必要性是多么重要？
影响因子：
23.9
作者：
Plaks, Vicki;Kong, Niwen;Werb, Zena
通讯作者：
Werb, Zena
DOI：
10.1039/c2ib00106c
发表时间：
2012-03
期刊：
Integrative biology : quantitative biosciences from nano to macro
影响因子：
--
作者：
Scherber C;Aranyosi AJ;Kulemann B;Thayer SP;Toner M;Iliopoulos O;Irimia D
通讯作者：
Irimia D
DOI：
10.1039/b908595e
发表时间：
2009-09
期刊：
Integrative biology : quantitative biosciences from nano to macro
影响因子：
--
作者：
Irimia D;Toner M
通讯作者：
Toner M
影响因子：
10.3
作者：
Rasheed, Zeshaan A.;Yang, Jie;Matsui, William
通讯作者：
Matsui, William