Rough deal and Zw10 are required for the metaphase checkpoint in Drosophila
Rough deal and Zw10 are required for the metaphase checkpoint in Drosophila
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DOI：
10.1038/35046592
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发表时间：
2000-12-01
影响因子：
21.3
通讯作者：
Karess, RE
中科院分区：
文献类型：
--
作者：
Basto, R;Gomes, R;Karess, RE
有丝分裂过程中的中期-后期转换受到精心调控，以确保遗传信息向子细胞的高保真传输。被称为中期检查点（或纺锤体组装检查点）的监视机制监测着丝粒与纺锤体微管的附着，并抑制后期开始，直到所有染色体在纺锤体上实现正确的双极取向。在这个检查点的缺陷导致过早的后期开始，并因此大大增加非整倍体的比率。在这里，我们表明，果蝇动粒组件粗糙的交易（杆）和Zeste-White 10（ZW 10）所需的中期检查点在苍蝇的正常运作。缺乏ROD或ZwlO的果蝇细胞表现出与bub 1突变体相似的表型-它们不会响应纺锤体损伤而停滞在中期，而是分离姐妹染色单体，降解细胞周期蛋白B并退出有丝分裂。这些是第一个被鉴定的检查点成分，在芽殖酵母中没有明显的同源物。
The metaphase-anaphase transition during mitosis is carefully regulated in order to assure high-fidelity transmission of genetic information to the daughter cells. A surveillance mechanism known as the metaphase checkpoint (or spindle-assembly checkpoint) monitors the attachment of kinetochores to the spindle microtubules, and inhibits anaphase onset until all chromosomes have achieved a proper bipolar orientation on the spindle. Defects in this checkpoint lead to premature anaphase onset, and consequently to greatly increased rates of aneuploidy. Here we show that the Drosophila kinetochore components Rough deal (Rod) and Zeste-White 10 (Zw10) are required for the proper functioning of the metaphase checkpoint in flies. Drosophila cells lacking either ROD or ZwlO exhibit a phenotype that is similar to that of bub1 mutants - they do not arrest in metaphase in response to spindle damage, but instead separate sister chromatids, degrade cyclin B and exit mitosis. These ave the first checkpoint components to be identified that do not have obvious homologues in budding yeast.