Primary structure of a novel gonadotropin-releasing hormone (GnRH) in the ovary of red swamp crayfish Procambarus clarkii
Primary structure of a novel gonadotropin-releasing hormone (GnRH) in the ovary of red swamp crayfish Procambarus clarkii
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克氏原螯虾卵巢中新型促性腺激素释放激素（GnRH）的一级结构
DOI：
10.1016/j.aquaculture.2013.10.010
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发表时间：
2014-01-01
期刊：
影响因子：
4.5
通讯作者：
Zhou, Xin
中科院分区：
文献类型：
--
作者：
Guan, Zheng-Bing;Shui, Yan;Zhou, Xin
促性腺激素释放激素（GnRH）是一种神经肽，已知调节和维持生殖功能，并在脊椎动物和无脊椎动物物种中保守。本研究利用层析和免疫学方法从克氏原螯虾卵巢中分离到一种促性腺激素释放激素（GnRH）肽。通过化学测序和质谱法确定其一级结构，证明该形式是GnRH的新形式，并命名为克氏毕赤酵母GnRH（pcGnRH）。pcGnRH的一级结构为pQSYHFSLGWKP-NH 2，其不同于脊椎动物和无脊椎动物GnRH家族的已知形式。合成的pcGnRH具有生物活性，对克氏原螯虾卵巢成熟有促进作用。我们的数据支持了这一假设，即在甲壳类动物中，有GnRH在卵巢局部合成，并在卵母细胞发育的调节中发挥重要作用。这是首次从甲壳动物中分离并鉴定GnRH样肽。皇冠版权所有（C）2013由爱思唯尔B. V.出版保留所有权利。
Gonadotropin-releasing hormone (GnRH) is a neuropeptide known to regulate and maintain reproductive functions and conserved in both vertebrate and invertebrate species. In this study, a GnRH peptide was isolated from the ovaries of red swamp crayfish, Procambarus clarkii, by using chromatographic and immunological methods. The form was proven to be a novel form of GnRH by determining its primary structure by chemical sequencing and mass spectrometry, and was designated as P. clarkii GnRH (pcGnRH). The primary structure of pcGnRH is pQSYHFSLGWKP-NH2, which is different from the known forms of the vertebrate and invertebrate GnRH family. Synthetic pcGnRH was biologically active, as it showed stimulative effects on ovarian maturation to crayfish. Our data supported the hypothesis that in crustacean there is GnRH synthesized locally in the ovary and playing an important role in the regulation of oocyte development. This is the first report on the isolation and characterization of GnRH-like peptide in crustacean. Crown Copyright (C) 2013 Published by Elsevier B.V. All rights reserved.