Virus infection induces the assembly of coordinately activated transcription factors on the IFN-β enhancer in vivo
Virus infection induces the assembly of coordinately activated transcription factors on the IFN-β enhancer in vivo
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DOI：
10.1016/s1097-2765(00)80051-9
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发表时间：
1998-03-01
期刊：
影响因子：
16
通讯作者：
Maniatis, T
中科院分区：
文献类型：
--
作者：
Wathelet, MG;Lin, CH;Maniatis, T
We have identified a virus-activated factor (VAF) that binds to a regulatory element shared by different virus-inducible genes. We provide evidence that VAF contains two members of the interferon regulatory factor (IRF) family of transcriptional activator proteins (IRF-3 and IRF-7), as well as the transcriptional coactivator proteins p300 and CBP. Remarkably, VAF, as well as recombinant IRF-3 and IRF-7 proteins, binds very weakly to the interferon-beta (IFN-beta) gene promoter in vitro. However, in virus-infected cells, both proteins are recruited to the endogenous IFN-beta promoter as part of a protein complex that includes ATF-S/c-Jun and NF-kappa B. These observations provide a unique example of the coordinate activation of multiple transcriptional activator proteins and their highly cooperative assembly into a transcriptional enhancer complex in vivo.
