Systematic test of neurotoxin dose and volume on muscle function in a rat model.
Systematic test of neurotoxin dose and volume on muscle function in a rat model.
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DOI：
10.1002/mus.23983
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发表时间：
2014-05
期刊：
影响因子：
3.4
通讯作者：
Lieber RL
中科院分区：
文献类型：
--
作者：
Hulst JB;Minamoto VB;Lim MB;Bremner SN;Ward SR;Lieber RL
肉毒杆菌毒素血清型A（BT-A）用于与异常肌肉活动相关的各种运动和感觉障碍。我们开发了一种高分辨率啮齿动物模型，以精确测定BT-A剂量（以单位测量）和注射液体积（以μL测量）对注射有效性和全身副作用的影响。背屈是注射和对侧肌肉功能的最佳指标。注射BT-A后1个月，各实验组注射BT-A的肢体背屈力矩均明显低于生理盐水对照组（P<0.05）。还比较了BT-A组之间的扭矩，这表明剂量有显著影响（P<0.001），但体积无影响（P>0.2），也无剂量x体积相互作用（P>0.3）。对于测量的其他参数，观察到类似的结果。这些数据表明，注射剂量而不是体积或浓度是啮齿动物模型中神经毒素功效的主要决定因素。
Botulinum toxin serotype A (BT-A) is used for a variety of motor and sensory disorders related to abnormal muscle activity. We developed a high-resolution rodent model to allow precise determination of the effect of BT-A dose (measured in units) and injectate volume (measured in μL) on the efficacy of the injection and systemic side effects. Dorsiflexion is the best indicator of injected and contralateral muscle function. One month after injection, dorsiflexion torque of BT-A-injected limbs was decreased significantly in all experimental groups compared with saline controls (P<0.05). Torque was also compared among the BT-A groups, which demonstrated a significant effect of dose (P<0.001), but no effect of volume (P>0.2) and no dose x volume interaction (P>0.3). Similar results were observed for other parameters measured. These data demonstrate that injection dose and not volume or concentration is the primary determinant of neurotoxin efficacy in a rodent model.