Hepatic de novo lipogenesis in normoinsulinemic and hyperinsulinemic subjects consuming high-fat, low-carbohydrate and low-fat, high-carbohydrate isoenergetic diets
Hepatic de novo lipogenesis in normoinsulinemic and hyperinsulinemic subjects consuming high-fat, low-carbohydrate and low-fat, high-carbohydrate isoenergetic diets
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DOI：
10.1093/ajcn/77.1.43
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发表时间：
2003-01-01
影响因子：
7.1
通讯作者：
Aghajanian, K
中科院分区：
文献类型：
--
作者：
Schwarz, JM;Linfoot, P;Aghajanian, K
背景：高胆固醇血症与心血管疾病风险增加相关。直到最近，由于方法学上的局限性，肝脏新生脂肪生成（DNL）在高脂血症中的重要性还难以评估。目的：我们评估了DNL对高脂血症相关的不同疾病的贡献程度。设计：经过5天的等能量高脂肪，低碳水化合物饮食，在正常胰岛素血症（小于或等于85 pmol/L）的瘦型（n = 9）和肥胖型（n = 6）以及高胰岛素血症（大于或等于115 pmol/L）肥胖型（n = 8）受试者中测量空腹DNL。在正常胰岛素血症瘦型（n = 5）和高胰岛素血症肥胖（n = 5）受试者中，在低脂、高碳水化合物饮食后测量空腹DNL。质量同位素分布分析被用来测量新合成的脂肪酸在VLDL-triacylglycerol.Results的分数：与高脂肪，低碳水化合物饮食，高胰岛素血症的肥胖受试者有一个3.7-5.3倍高分数DNL（8.5 +/- 0.7%）比normoinsulinemic瘦（1.6 +/- 0.5%）或肥胖（2.3 +/- 0.3%）的主题。在低脂、高碳水化合物饮食的情况下，正常胰岛素血症的瘦型和高胰岛素血症的肥胖受试者具有相似的高DNL分数（分别为13 +/- 5.1%和12.8 +/-1.4%）。与基线相比，高脂肪、低碳水化合物饮食的消耗并不影响三酰甘油浓度。然而，。在低脂肪、高碳水化合物饮食后，三酰基甘油显著增加，DNL比食用高脂肪饮食的正常胰岛素血症受试者高5-6倍。低脂、高碳水化合物饮食后甘油三酯的增加与DNL分数相关（P < 0.01），表明高DNL受试者的甘油三酯增加最多。结论：这些结果支持高胰岛素血症和低脂饮食均增加DNL的概念，DNL有助于高胰岛素血症。
Background: Hypertriglyceridemia is associated with increased risk of cardiovascular disease. Until recently, the importance of hepatic de novo lipogenesis (DNL) in contributing to hypertriglyceridemia was difficult to assess because of methodologic limitations.Objective: We evaluated the extent of the contribution by DNL to different conditions associated with hypertriglyceridemia.Design: After 5 d of an isoenergetic high-fat, low-carbohydrate diet, fasting DNL was measured in normoinsulinemic (less than or equal to85 pmol/L) lean (n = 9) and obese (n = 6) and hyperinsulinemic (greater than or equal to 115 pmol/L) obese (n = 8) subjects. Fasting DNL was measured after a low-fat, high-carbohydrate diet in normoinsulinemic lean (n = 5) and hyperinsulinemic obese (n = 5) subjects. Mass isotopomer distribution analysis was used to measure the fraction of newly synthesized fatty acids in VLDL-triacylglycerol.Results: With the high-fat, low-carbohydrate diet, hyperinsulinemic obese subjects had a 3.7-5.3-fold higher fractional DNL (8.5 +/- 0.7%) than did normoinsulinemic lean (1.6 +/- 0.5%) or obese (2.3 +/- 0.3%) subjects. With the low-fat, high-carbohydrate diet, normoinsulinemic lean and hyperinsulinemic obese subjects had similarly high fractional DNL (13 +/- 5.1% and 12.8 +/- 1.4%, respectively). Compared with baseline, consumption of the high-fat, low-carbohydrate diet did not affect triacylglycerol concentrations. However,. after the low-fat, high-carbohydrate diet, triacylglycerols increased significantly and DNL was 5-6-fold higher than in normoinsulinemic subjects consuming a high-fat diet. The increase in triacylglycerol after the low-fat, high-carbohydrate diet was correlated with fractional DNL (P < 0.01), indicating that subjects with high DNL had the greatest increase in triacylglycerols.Conclusions: These results support the concept that both hyperinsulinemia and a low-fat diet increase DNL, and that DNL contributes to hypertriglyceridemia.