Establishing a genetic system for ecological studies of Streptococcus oligofermentans.
Establishing a genetic system for ecological studies of Streptococcus oligofermentans.
复制标题
建立寡发酵链球菌生态研究的遗传系统。
DOI：
10.1111/j.1574-6968.2006.00453.x
复制
发表时间：
2006
影响因子：
2.1
通讯作者：
Dong,Xiuzhu
中科院分区：
文献类型：
--
作者：
Tong,Huichun;Zhu,Baoli;Chen,Wei;Qi,Fengxia;Shi,Wenyuan;Dong,Xiuzhu
寡链球菌发酵菌是口腔链球菌属的一种新的特定种。到目前为止，还没有证据表明两者之间存在相关性。寡聚发酵剂和龋齿。此外，还观察到了OF数量之间的负相关关系。口腔中主要的致龋性病原体--变形链球菌的数量和寡发酵。这些特性说明了这一点。寡聚发酵剂有可能被用作预防龋病的“益生菌”。在本研究中，我们的目标是建立INS的遗传系统。以进一步研究这一新物种的生物学。利用其他链球菌的comCDE基因座的同源区域，分离并测序了comC基因。合成了一种合成的能力刺激肽(CSP)，并证明它能有效地诱导能力INS。寡聚发酵菌。这个CSP诱导的转化系统是INS。利用寡核苷酸发酵构建了由乳酸脱氢酶(LDH)启动子驱动的绿色荧光蛋白(GFP)和荧光素酶(Luc)报告系统。进一步证明这些报告系统在浮游细胞和生物膜细胞中高度表达，这表明这些报告系统可以用于未来的生态学研究。寡聚发酵菌。
Streptococcus oligofermentansis a newly characterized species belonging to the mitis group of oral streptococci. So far no correlation has been demonstrated betweenS. oligofermentansand dental caries. Furthermore, a reverse correlation has been observed between the number ofS. oligofermentansand the number ofStreptococcus mutans, a major cariogenic pathogen, in the oral cavity. These properties suggest thatS. oligofermentansmay have a potential to be used as a ‘probiotics’ for caries prevention. In this study, we aim to establish a genetic system inS. oligofermentansto further study the biology of this new species. Using homologous regions of thecomCDElocus in other streptococci, thecomCgene was isolated and sequenced. A synthetic competence-stimulating peptide (CSP) was synthesized and shown to be able to effectively induce competence inS. oligofermentans. This CSP-induced transformation system inS. oligofermentanswas used to construct green fluorescent protein (gfp) and luciferase (luc) reporter systems, both of which are driven by the lactate dehydrogenase (ldh) promoter. These reporter systems were further shown to be highly expressed in planktonic and biofilm cells, suggesting that these reporter systems can be used in future ecological studies ofS. oligofermentans.