NEURAL EXPRESSION AND CHROMOSOMAL MAPPING OF NEU DIFFERENTIATION FACTOR TO 8P12-P21
NEURAL EXPRESSION AND CHROMOSOMAL MAPPING OF NEU DIFFERENTIATION FACTOR TO 8P12-P21
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DOI：
10.1073/pnas.90.5.1867
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发表时间：
1993-03-01
影响因子：
11.1
通讯作者：
YARDEN, Y
中科院分区：
文献类型：
--
作者：
ORRURTREGER, A;TRAKHTENBROT, L;YARDEN, Y
Neu分化因子(NDF/hereglin)是一种44 kDa的糖蛋白，与Neu/ErbB-2受体酪氨酸激酶相互作用，增加其在酪氨酸残基上的磷酸化。在体外，NDF促进某些乳腺肿瘤细胞向产奶细胞的分化。作为了解NDF的生理作用的第一步，我们进行了原位杂交分析，以确定mRNAs在小鼠胚胎中的分布，并将该基因定位于人类核型。在性交后14.5天的小鼠胚胎中，NDF的表达主要局限于中枢和外周神经系统，包括排列在侧脑室、脊髓腹角、肠道和背根节的神经上皮细胞。其他含有NDF转录本的组织包括肾上腺、肝脏以及真皮和生发脊的不同细胞层。H-3标记的探针与人中期分裂相的原位杂交将NDF基因定位于8号染色体短臂的p12-p21带。
Neu differentiation factor (NDF/heregulin) is a 44-kDa glycoprotein that interacts with the Neu/ErbB-2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues. In vitro NDF promotes differentiation of certain mammary tumor cell lines to milk-producing cells. As a first step toward understanding the physiological role of NDF, we performed in situ hybridization analyses to determine mRNA distribution in the mouse embryo and to map the gene to human karyotypes. In 14.5-day-postcoitum mouse embryos, NDF expression is confined predominantly to the central and peripheral nervous system, including the neuroepithelium that lines the lateral ventricles of the brain, the ventral horn of the spinal cord, and the intestinal as well as dorsal root ganglia. Other tissues that contain NDF transcripts are the adrenal gland, liver, and distinct cell layers of the dermis and germinal ridge. In situ hybridization of a H-3-labeled probe to human metaphase spreads localized the NDF gene to the short arm of chromosome 8 at bands p12-p21.