One-step cascade detection of glucose at neutral pH based on oxidase-integrated copper(II) metal-organic framework composites
One-step cascade detection of glucose at neutral pH based on oxidase-integrated copper(II) metal-organic framework composites
复制标题
基于氧化酶集成铜(ii)金属有机框架复合材料的中性pH下葡萄糖的一步级联检测
DOI：
10.1039/d0nj02550j
复制
发表时间：
2020-08-07
影响因子：
3.3
通讯作者：
Li, Yongxin
中科院分区：
文献类型：
--
作者：
Huang, Hui;Zhang, Wenjing;Li, Yongxin
由铜 (II) 金属有机框架 (Cu-MOF) 和葡萄糖氧化酶 (GOx) 构建了一个集成系统，用于在中性 pH 值（pH = 7.0）下对葡萄糖进行一步级联测定。在此，Cu-MOF 具有负载 GOx 和模拟过氧化物酶的双重功能。 GOx@MOF集成复合材料是在温和条件下合成的。在集成系统中，葡萄糖在氧气存在下被 GOx 氧化成葡萄糖酸和过氧化氢。在MOF催化剂存在下，过氧化氢作为中间产物，立即转化为(OH)-O-中心点，氧化2,2'-连氮基-双(3-乙基苯并噻唑啉-6-磺酸)(ABTS)，并在420 nm处观察到紫外-可见吸光度。葡萄糖浓度与吸光度在8-140μM范围内呈正线性相关，检测限为2.67μM。我们的策略具有准确度高、易于操作和生物应用的特点。已成功应用于人血清中葡萄糖的检测，为生物分析提供了更加便捷、灵敏的操作平台。
An integrated system was fabricated from a copper(II) metal-organic framework (Cu-MOF) and glucose oxidase (GOx) for one-step cascade determination of glucose at neutral pH (pH = 7.0). Herein, the Cu-MOF performs dual functions of loading GOx and mimicking peroxidase. The GOx@MOF integrated composites were synthesized under mild conditions. In the integrated system, glucose was oxidized to gluconic acid and hydrogen peroxide by GOx in the presence of oxygen. In the presence of the MOF catalyst, hydrogen peroxide, acting as an intermediate product, was immediately converted to (OH)-O-center dot, which oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and UV-vis absorbance at 420 nm was observed. The glucose concentration is positively and linearly correlated with absorbance in the range 8-140 mu M with a detection limit of 2.67 mu M. Our strategy possesses the characteristics of excellent accuracy, easy operation and biological application. It has been successfully applied to the detection of glucose in human serum, and provides a more convenient and sensitive operation platform for biological analysis.