Photo-induced hydrophilicity enhances initial cell behavior and early bone apposition
Photo-induced hydrophilicity enhances initial cell behavior and early bone apposition
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DOI：
10.1111/j.1600-0501.2007.01509.x
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发表时间：
2008-05-01
影响因子：
4.3
通讯作者：
Atsuta, Mitsuru
中科院分区：
文献类型：
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作者：
Sawase, Takashi;Jimbo, Ryo;Atsuta, Mitsuru
目的：纳米二氧化钛（TiO 2）在紫外光照射下表现出光诱导亲水性。材料与方法：采用等离子体源离子注入法，在钛片和种植体表面包覆纳米TiO 2，并经后退火处理。一半的盘和植入物预先用UV照射24小时，而另一半是盲的并用作对照。通过静态润湿性测定证实了光诱导的亲水性。这种亲水性对细胞行为的影响通过使用多能间充质前体C2 C12细胞的细胞附着、增殖和形态学来评估。此后，骨形成周围的亲水性植入物插入在兔胫骨histomorphometric.Results确认：光诱导的亲水磁盘的水接触角显着下降，从43.5度到0.5度。细胞附着和增殖在这种亲水性的磁盘上显示出显着的改善。在该亲水性盘上的细胞形态极其扁平，具有板状伪足的伸长，而在对照盘上观察到圆形/球形形态。光诱导亲水性种植体促进了骨形成，愈合2周后骨与金属的接触率为28.2%（对照组：17.97%）。结论：本研究中使用的光诱导亲水性表面改善了初始细胞反应，并促进了早期骨与种植体的沉积。
Objective: The anatase form of titanium dioxide (TiO2) exhibits photo-induced hydrophilicity when it is irradiated with ultraviolet (UV) light. In the present study, the effect of photo-induced hydrophilicity on initial cell behavior and bone formation was evaluated.Materials and methods: Plasma source ion implantation method and post-annealing were employed for coating the anatase form of TiO2 to the surface of the titanium disk and implant. Half of the disks and implants were illuminated with UV for 24 h beforehand, whereas the other halves were blinded and used as controls. Photo-induced hydrophilicity was confirmed by a static wettability assay. The effects of this hydrophilicity on cell behavior were evaluated by means of cell attachment, proliferation and morphology using pluripotent mesenchymal precursor C2C12 cells. Thereafter, bone formation around the hydrophilic implant inserted in the rabbit tibia was confirmed histomorphometrically.Results: The water contact angle of the photo-induced hydrophilic disk decreased markedly from 43.5 degrees to 0.5 degrees. Cell attachment and proliferation on this hydrophilic disk showed significant improvement. The cell morphology on this hydrophilic disk was extremely flattened, with an elongation of the lamellipodia, whereas a round/spherical morphology was observed on the control disk. The photo-induced hydrophilic implant enhanced the bone formation with the bone-to-metal contact of 28.2% after 2 weeks of healing (control: 17.97%).Conclusion: The photo-induced hydrophilic surface used in the current study improves the initial cell reactions and enhances early bone apposition to the implant.