Apigenin inhibits African swine fever virus infection in vitro
Apigenin inhibits African swine fever virus infection in vitro
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DOI：
10.1007/s00705-016-3061-y
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发表时间：
2016-12-01
影响因子：
2.7
通讯作者：
Zakaryan, Hovakim
中科院分区：
文献类型：
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作者：
Hakobyan, Astghik;Arabyan, Erik;Zakaryan, Hovakim
非洲猪瘟病毒（ASFV）是对家猪最具破坏性的疾病之一，目前尚无有效疫苗。黄酮类化合物是一种从植物中分离出来的天然产物，据报道，黄酮类化合物在体内和体外对不同的病毒具有显著的抗病毒活性。在这里，我们测试了五种黄酮类化合物对Vero细胞中ASFV复制的抗病毒作用。我们的研究结果表明，芹菜素在体外具有强效的剂量依赖性抗asfv作用。添加时间实验表明，芹菜素在感染的早期阶段非常有效。当添加1 hpi时，芹菜素可使ASFV产量降低99.99%以上。通过对ASFV蛋白合成和病毒工厂的评价，进一步研究了芹菜素的抗病毒活性。黄酮类化合物抑制asfv特异性蛋白的合成和病毒工厂的形成。持续用芹菜素处理的asfv感染细胞未显示细胞病变效应。需要进一步研究芹菜素在体内的应用。
African swine fever virus (ASFV) is one of the most devastating diseases of domestic pigs for which no effective vaccines are available. Flavonoids, natural products isolated from plants, have been reported to have significant in vitro and in vivo antiviral activity against different viruses. Here, we tested the antiviral effect of five flavonoids on the replication of ASFV in Vero cells. Our results showed a potent, dose-dependent anti-ASFV effect of apigenin in vitro. Time-of-addition experiments revealed that apigenin was highly effective at the early stages of infection. Apigenin reduced the ASFV yield by more than 99.99 % when it was added at 1 hpi. The antiviral activity of apigenin was further investigated by evaluation of ASFV protein synthesis and viral factories. This flavonoid inhibited ASFV-specific protein synthesis and viral factory formation. ASFV-infected cells continuously treated with apigenin did not display a cytopathic effect. Further studies addressing the use of apigenin in vivo are needed.