Assessment of cellular viability on calcium sulphate/hydroxyapatite injectable scaffolds.
Assessment of cellular viability on calcium sulphate/hydroxyapatite injectable scaffolds.
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DOI：
10.1177/2041731413509645
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发表时间：
2013
影响因子：
8.2
通讯作者：
Ayoub A
中科院分区：
文献类型：
--
作者：
Alfotawi R;Naudi K;Dalby MJ;Tanner KE;McMahon JD;Ayoub A
本文对旋转肌钙化修复颌骨缺损的骨水泥进行了试验。本研究的目的是研究两种市售骨水泥（Cerament™脊柱支架和Cerament骨空隙填充物）与间充质细胞和细胞因子（骨形态发生蛋白）的负载可见性，以作为未来临床应用的仿生支架。使用甲基噻唑基四唑测定基本生物相容性（细胞活力），并在不同时间点使用MG-63细胞进行活/死测定。接下来，为了告知潜在的后续体内实验，使用胶原组织模拟物通过免疫荧光细胞骨架染色表征兔间充质基质细胞，并同时然后依次将Cerament Spine Support骨水泥和细胞注射到胶原凝胶中。结果表明，Cerament脊柱支架具有更好的生物相容性，将骨水泥和兔间充质基质细胞顺序注射到组织模拟物中是临床应用的最佳方法。
Cements for maxillofacial reconstruction of jaw defects through calcification of rotated muscle have been tested. The objective of this study was to investigate the visibility of loading of two types of commercially available cements, Cerament™ Spine Support and Cerament Bone Void Filler with mesenchymal cells and cytokines (bone morphogenetic protein) to act as a biomimetic scaffolding for future clinical application. Determination of basic biocompatibility (cell viability) using methyl thiazolyl tetrazolium and live/dead assay was carried out using MG-63 cells at various time points. Next, in order to inform potential subsequent in vivo experiments, a collagen tissue mimic was used for characterization of rabbit mesenchymal stromal cells using immunofluorescent cytoskeleton staining, and simultaneous and then sequential injection of Cerament Spine Support cement and cells into collagen gels. Results indicated that Cerament Spine Support was more biocompatible and that sequential injection of cement and then rabbit mesenchymal stromal cells into the tissue mimics is an optimal approach for clinical applications.