The effect of oxytocin and Kisspeptin-10 in ovary and uterus of ischemia-reperfusion injured rats
The effect of oxytocin and Kisspeptin-10 in ovary and uterus of ischemia-reperfusion injured rats
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DOI：
10.1016/j.tjog.2016.12.018
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发表时间：
2017-08-01
影响因子：
2.1
通讯作者：
Yilmaz, B.
中科院分区：
文献类型：
--
作者：
Aslan, M.;Senturk, G. Erkanli;Yilmaz, B.
目的：缺血/再灌注（I/R）损伤导致内皮细胞和实质细胞损伤。催产素 (OXY) 刺激分娩期间的子宫收缩和哺乳期间的肌上皮细胞。 OXY 已被用作保护性抗氧化剂。 Kisspeptin 在生殖功能和青春期开始的中央控制中发挥着关键作用。最近的研究表明，这些生殖激素具有抗氧化剂的保护潜力。本研究的目的是探讨Kisspeptin和OXY作为抗氧化剂对雌性大鼠I/R损伤的卵巢和子宫的潜在保护作用。材料和方法：将大鼠分为五组。第1组，为对照组；第2组，对大鼠进行缺血，然后再灌注。第3组，在I/R施用大鼠之前30分钟施用OXY；第4组，在I/R施用大鼠之前30分钟施用Kisspeptin；第 5 组，在 I/R 前 30 分钟施用 OXY 和 Kisspeptin。取出卵巢和子宫进行组织病理学和生化观察。分析丙二醛、谷胱甘肽水平和超氧化物歧化酶活性，以观察 OXY 和 Kisspeptin 的抗氧化潜力。苏木精和伊红染色进行组织病理学评分。结果：I/R组卵巢间质细胞和颗粒细胞、子宫内膜细胞受损。与仅注射 Kisspeptin 的 I/R 组和 I/R 组相比，OXY 和 Kisspeptin 给药的 I/R 组卵巢和子宫的细胞损伤减少。 OXY 和 OXY Kisspeptin 注射 I/R 组之间没有显着差异。与 I/R 组相比，应用 Kisspeptin 和/或催产素 I/12 组的 MDA 水平降低。与I/R组相比，应用Kisspeptin和/或OXY的I/R组SOD活性和GSH水平升高。结论：本研究结果表明，外源性应用催产素和Kisspeptin对子宫和卵巢具有抗氧化作用。 (C) 2017 台湾妇产科学会。 Elsevier B.V. 的出版服务
Objective: ischemia/reperfusion (I/R) injuries result in damage to endothelial and parenchymal cells. Oxytocin (OXY) stimulates uterine contraction during parturition and myoepithelial cells during suckling. OXY has been used as a protective antioxidant. Kisspeptin plays a key role in the central control of reproductive functions and onset of puberty. Recent studies show that these reproductive hormones have protective potential as antioxidant. The aim of this study is to investigate the potential protective effects of Kisspeptin and OXY as antioxidants on I/R injured ovary and uterus of female rats.Materials and methods: Rats were separated into five groups. Group 1, is control group; Group 2, rats were subjected to ischemia followed by reperfusion. Group 3, OXY administration 30 min prior to I/R applied rats; Group 4, Kisspeptin administration 30 min prior to I/R applied rats; Group 5, OXY and Kisspeptin administration 30 min prior to I/R. Ovary and uterus were removed for histopathological and biochemical observations. Malondialdehyde, glutathione levels, and superoxide dismutase activities were analyzed in order to observe antioxidant potential of OXY and Kisspeptin. Hematoxylin and Eosin staining was applied for histopathologic scoring.Results: Stromal and granulosa cells in ovary, endometrial cells in uterus were damaged in I/R group. The cellular damage of ovary and uterus were reduced in OXY and Kisspeptin administered I/R group when compared to only Kisspeptin injected I/R group and I/R group. There is no significant difference between OXY and OXY Kisspeptin injected I/R groups. MDA levels were decreased in Kisspeptin and/or Oxytocin applied I/12 group compared to I/R group. SOD activity and GSH levels were increased in Kisspeptin and/or OXY applied I/R group compared to I/R group.Conclusions: The present results suggest that exogenous application of oxytocin and kisspeptin can have antioxidant effects on the uterus and ovary. (C) 2017 Taiwan Association of Obstetrics & Gynecology. Publishing services by Elsevier B.V.