High-Performance Capillary Electrophoresis for Determining HIV-1 Tat Protein in Neurons
High-Performance Capillary Electrophoresis for Determining HIV-1 Tat Protein in Neurons
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DOI：
10.1371/journal.pone.0016148
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发表时间：
2011-01-07
期刊：
影响因子：
3.7
通讯作者：
Sawaya, Bassel E.
中科院分区：
文献类型：
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作者：
Deshmane, Satish L.;Mukerjee, Ruma;Sawaya, Bassel E.
HIV-1蛋白Tat与艾滋病的发病机制有关，然而，人们认为循环Tat的数量非常低，其定量也很困难。我们使用高效毛细管电泳对感染细胞释放并被神经元吸收的Tat进行了定量分析。这是第一份成功测量神经元中Tat数量的报告，并将Tat作为hiv相关神经认知障碍的关键参与者。
The HIV-1 protein, Tat has been implicated in AIDS pathogenesis however, the amount of circulating Tat is believed to be very low and its quantification has been difficult. We performed the quantification of Tat released from infected cells and taken up by neurons using high performance capillary electrophoresis. This is the first report to successfully measure the amount of Tat in neurons and places Tat as a key player involved in HIV-associated neurocognitive disorders.