Rat survival to anthrax lethal toxin is likely controlled by a single gene
Rat survival to anthrax lethal toxin is likely controlled by a single gene
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DOI：
10.1038/sj.tpj.6500448
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发表时间：
2008-02-01
影响因子：
2.8
通讯作者：
Jacob, H. J.
中科院分区：
文献类型：
--
作者：
Nye, S. H.;Wittenburg, A. L.;Jacob, H. J.
我们研究了给予炭疽致死毒素的不同鼠种的存活率是否是由基因决定的。根据祖先对炭疽致死毒素的敏感性和最大限度地提高品系间的遗传多样性，培育了第一代杂交大鼠的可重复试验种群。然后，这些老鼠被测试了不同剂量的炭疽致命毒素。我们发现，所有的“敏感”菌株死亡后2小时内全身给药240微克/公斤致死毒素，而一个菌株存活后，5倍的剂量（1.4毫克/公斤）。致死毒素裂解来自这些菌株骨髓的巨噬细胞培养物的能力与体内结果一致。我们的结论是，大鼠测试人口可以检测菌株的差异，炭疽致死毒素。存活率受宿主基因组背景的影响，并且可能是由于具有隐性遗传模式的单个基因。
We examined whether survival of different rat strains administered anthrax lethal toxin is genetically determined. A reproducible test population of first filial generation hybrid rats was bred based on the susceptibility of progenitors to anthrax lethal toxin and to maximize genetic diversity across the strains. These rats were then tested with varying doses of anthrax lethal toxin. We found that all 'sensitive' strains died within 2 h following systemic administration of 240 mu g/kg lethal toxin, while one strain survived following a five times higher dose (1.4 mg/kg). The ability of lethal toxin to lyse macrophage cultures derived from the bone marrow of these strains corresponded with in vivo results. We conclude that a rat test population can detect strain differences in response to anthrax lethal toxin. Survival is influenced by the host genome background and is likely due to a single gene with a recessive mode of inheritance.