A practical model of osteomyelitis-induced bone pain by intra-tibial injection of Staphylococcus aureus in rats
A practical model of osteomyelitis-induced bone pain by intra-tibial injection of Staphylococcus aureus in rats
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DOI：
10.1016/j.neulet.2012.02.038
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发表时间：
2012-04
影响因子：
2.5
通讯作者：
Chang-Jiang Yang;Qian Li;Gen-cheng Wu;Yan-Qing Wang;Q. Mao-Ying
中科院分区：
文献类型：
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作者：
Chang-Jiang Yang;Qian Li;Gen-cheng Wu;Yan-Qing Wang;Q. Mao-Ying
骨髓炎是一种急性或慢性骨炎症过程，伴有轻度至重度疼痛。一般来说，骨髓炎引起的轻至中度疼痛可以缓解，但重度疼痛则不能缓解。因此，需要进一步研究骨髓炎引起的剧烈疼痛的机制。在本研究中，通过胫骨内注射金黄色葡萄球菌来诱导传统的大鼠骨髓炎模型。然后，进行了一系列测试，包括骨组织学、血液分析、机械异常性疼痛、热痛觉过敏和免疫组织化学。胫骨内注射细菌 4 天后，骨髓中出现急性炎症，12 天后发展为慢性炎症。血液分析结果证实了骨炎症的存在。注射后不久出现明显的机械异常性疼痛和热痛觉过敏。这种骨髓炎引起的疼痛行为可以被塞来昔布（一种选择性 COX-2 抑制剂）逆转。此外，在脊髓中观察到小胶质细胞和星形胶质细胞显着增加。我们的结果表明，骨髓炎诱导的大鼠表现出与疼痛相关的行为和相关的神经化学变化。因此，本研究提供了一种新颖的骨炎症引起疼痛的实用大鼠模型。
Osteomyelitis is an acute or chronic inflammatory process of bone accompanied with mild to severe pain. Generally, mild to moderate pain induced by osteomyelitis can be relieved, yet severe pain cannot. Therefore, a further investigation into the mechanism of severe pain induced by osteomyelitis is needed. In this study, a traditional rat model of osteomyelitis was induced by intra-tibial injection of Staphylococcus aureus. Then, a series of tests including bone histology, blood analysis, mechanical allodynia, thermal hyperalgesia, and immunohistochemistry were performed. Four days after an intra-tibial bacterial injection, acute inflammation was observed in the bone marrow, which developed into chronic inflammation 12 days after the procedure. The results from the blood analysis confirmed the existence of bone inflammation. Significant mechanical allodynia and thermal hyperalgesia developed shortly after the injection. This osteomyelitis-induced pain behavior was reversed by Celecoxib, a selective COX-2 inhibitor. Furthermore, significant increase of both microglia and astrocytes was observed in the spinal cord. Our results suggest that osteomyelitis-induced rats display pain related behaviors and associated neurochemical changes. This study thus provides a novel practical rat model of bone inflammation induced pain.