Probing Micromechanical Properties of the Extracellular Matrix of Soft Tissues by Atomic Force Microscopy
Probing Micromechanical Properties of the Extracellular Matrix of Soft Tissues by Atomic Force Microscopy
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DOI：
10.1002/jcp.25420
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发表时间：
2017-01-01
影响因子：
5.6
通讯作者：
Navajas, Daniel
中科院分区：
文献类型：
--
作者：
Jorba, Ignasi;Uriarte, Juan J.;Navajas, Daniel
细胞外基质（ECM）决定了3D组织结构，并为细胞提供结构支持以及化学和机械提示。原子力显微镜（AFM）具有独特的能力来测量ECM力学的规模，在细胞探测其微环境的机械特征。此外，AFM测量可以很容易地与明场和荧光显微镜相结合。使用AFM进行可靠的机械测量需要对设备进行准确的校准并正确计算机械参数。将ECM机制与细胞贡献分离的合适方法是通过有效的脱细胞化过程去除细胞，该脱细胞化过程保留ECM的组成、结构和机械性质。ECM微观力学的AFM测量提供了重要的见解器官生物fabrics，细胞基质机械串扰和疾病引起的组织刚度改变。(C)2016 Wiley Periodicals，Inc.
The extracellular matrix (ECM) determines 3D tissue architecture and provides structural support and chemical and mechanical cues to the cells. Atomic force microscopy (AFM) has unique capabilities to measure ECM mechanics at the scale at which cells probe the mechanical features of their microenvironment. Moreover, AFM measurements can be readily combined with bright field and fluorescence microscopy. Performing reliable mechanical measurements with AFM requires accurate calibration of the device and correct computation of the mechanical parameters. A suitable approach to isolate ECM mechanics from cell contribution is removing the cells by means of an effective decellularization process that preserves the composition, structure and mechanical properties of the ECM. AFM measurement of ECM micromechanics provides important insights into organ biofabrication, cell-matrix mechanical crosstalk and disease-induced tissue stiffness alterations. (C) 2016 Wiley Periodicals, Inc.