Amperometric Hydrogen Peroxide Biosensor Based on Horseradish Peroxidase Immobilized on Fe3O4/Chitosan Modified Glassy Carbon Electrode
Amperometric Hydrogen Peroxide Biosensor Based on Horseradish Peroxidase Immobilized on Fe3O4/Chitosan Modified Glassy Carbon Electrode
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DOI：
10.1002/elan.200804572
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发表时间：
2009-07
期刊：
影响因子：
3
通讯作者：
Xuecai Tan;Jinlei Zhang;Sheng-Wei Tan;Dan-dan Zhao;Zenwei Huang;Yan Mi;Zaiyin Huang
中科院分区：
文献类型：
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作者：
Xuecai Tan;Jinlei Zhang;Sheng-Wei Tan;Dan-dan Zhao;Zenwei Huang;Yan Mi;Zaiyin Huang
将辣根过氧化物酶（HRP）固定在Fe 3 O 4/壳聚糖修饰玻碳电极上，构建了一种新型的电流型过氧化氢生物传感器。考察了支持电解质浓度、pH值、酶负载量、亚甲基蓝（MB）浓度和外加电位等实验因素对酶催化降解的影响。H2 O2校准曲线的线性范围为2.0×10−4-1.2×10−2 M，检出限为1.0×10−4 M（S/N=3）。响应时间小于12 s。表观米氏常数Km为21.4mM，说明固定化酶具有良好的生物活性。此外，该传感器具有长期稳定性和良好的重现性。该方法已用于真实的样品中H2 O2浓度的测定。
A new type of amperometric hydrogen peroxide biosensor was constructed based on horseradish peroxidase (HRP) immobilized on Fe3O4/chitosan modified glassy carbon electrode. The effects of some experimental variables such as the concentration of supporting electrolyte, pH, enzyme loading, the concentration of the mediator of methylene blue (MB) and the applied potential were investigated. The linear range of the calibration curve for H2O2 was 2.0×10−4–1.2×10−2 M with a detection limit of 1.0×10−4 M (S/N=3). The response time was less than 12 s. The apparent Michaelis-Menten constant Km was 21.4 mM and it illustrated the excellent biological activity of the fixed enzyme. In addition, the biosensor had long-time stability and good reproducibility. And this method has been used to determine H2O2 concentration in the real sample.