Establishment, characterization, karyotyping, and comparative genomic hybridization analysis of HKESC-2 and HKESC-3: two newly established human esophageal squamous cell carcinoma cell lines
Establishment, characterization, karyotyping, and comparative genomic hybridization analysis of HKESC-2 and HKESC-3: two newly established human esophageal squamous cell carcinoma cell lines
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DOI：
10.1016/s0165-4608(01)00580-5
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发表时间：
2002-06-01
影响因子：
--
通讯作者：
Srivastava, G
中科院分区：
文献类型：
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作者：
Hu, YC;Lam, KY;Srivastava, G
食管癌细胞系的建立有助于探索其发病的分子机制。两个新的人食道鳞状细胞癌细胞系。HKESC-2和HKESC-1。均由一名46岁中国女性的中分化ESCC和一名74岁中国男性的高分化ESCC建立，两者均来自香港。病理特征(形态、免疫组织化学)。和电子显微镜研究)。观察两种细胞系在裸鼠体内的致瘤性、细胞遗传学特征和DNA倍体。这两个细胞系已在体外培养超过17个月，HKESC-2传代超过85次，HKESC-3传代58次。两者均呈单层生长，HKESC-2和HKESC-3的倍增时间分别为24小时和48小时。通过抗细胞角蛋白抗体的强免疫阳性以及张力细丝和桥粒的超微结构显示，证实了它们的鳞状上皮性质。它们在裸鼠体内具有致瘤性，并具有DNA非整倍体。G显带细胞遗传学分析显示HKESC-2为超二倍体，HKESC-3为近四倍体。在1p22、1p32处出现频繁断点。在HKESC-2中为9q34，在1p31、3p25、3p14为6q16。6q21。8p21。9q34。13q32和17q25。比较基因组杂交分析发现，HKESC-2的染色体扩增分别位于3q24、5q21、8q11、13q21、q31、17q11、19、22q22和3q13-qter。5p 6p，9q21类似于qter，10q21类似于q22 12q15类似顶端。14q24类似于香港中学会考-3，16，17q24类似于20。HKESC-3的染色体丢失位于3p13类似的顶端，18q12类似的q12。这两个新建立的细胞系将为研究ESCC细胞的分子发病机制和生物学行为，以及未来测试新的ESCC治疗试剂提供有用的工具。(C)2002 Elsevier Science Inc.保留所有权利。
The establishment of esophageal cancer cell lines can facilitate the search for molecular mechanism underlying its pathogenesis. Two novel human esophageal squamous Cell carcinoma (ESCC) cell lines. HKESC-2 and HKESC-1. were established from a moderately differentiated ESCC of a 46-year-old Chinese woman and a well-differentiated ESCC of a 74-ycar-old Chinese man, both from Hong Kong. The pathological characteristics (morphological, immunohistochemical. and electron microscopic studies). tumorigenicity in nude mice, cytogenetic features, and DNA ploidy of the two cell lines were investigated. The two cell lines have been maintained in vitro for more than 17 months and passaged over 85 times for HKESC-2 and 58 times for HKESC-3. Both grew as monolayers, with a doubling time of 24 hours for HKESC-2 and 48 h for HKESC-3. Their squamous epithelial nature was authenticated by their strong immunopositivity with the anti-cytokeratin antibodies and the ultrastructural demonstration of tonofilaments and desmosomes. They are tumorigenic in nude mice and had DNA aneuploidy. G-banding cytolgenetic analysis showed hyperdiploidy in HKESC-2 and near-tetraploidy in HKESC-3. Frequent breakpoints were noted at 1p22, 1p32. and 9q34 in HKESC-2 and at 1p31, 3p25, 3p14 6q16. 6q21. 8p21. 9q34. 13q32, and 17q25 in HKESC-3. Comparative genomic hybridization analysis found that chromosomal gains were at 3q24similar toqter, 5q21similar toqter, 8q11similar toqter, 13q21similar toq31 17q11similar toqter, 19, 22q22 for HKESC-2 and at 3q13-qter. 5p 6p, 9q21similar toqter, 10q21similar toq22 12q15similar topter. 14q24similar toqter, 16, 17q24similar toqter 20 for HKESC-3. Chromosomal losses were at 3p13similar topter, 18q12similar toqter for HKESC-3. These two newly established cell lines will be useful tools in the study of the molecular pathogenesis and biological behavior of ESCC cells and for testing new therapeutic reagents for ESCC in the future. (C) 2002 Elsevier Science Inc. All rights reserved.