Analogs of LDL Receptor Ligand Motifs in Dengue Envelope and Capsid Proteins as Potential Codes for Cell Entry.
Analogs of LDL Receptor Ligand Motifs in Dengue Envelope and Capsid Proteins as Potential Codes for Cell Entry.
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DOI：
10.1155/2015/646303
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发表时间：
2015-05
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影响因子：
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通讯作者：
J. Guevara;Jamie Romo;Troy McWhorter;N. V. Guevara
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文献类型：
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作者：
J. Guevara;Jamie Romo;Troy McWhorter;N. V. Guevara
已证实含有载脂蛋白B100和载脂蛋白E的低密度脂蛋白颗粒(LLP)的细胞进入是由受体和GAG介导的。登革病毒1-4株的受体配体基序XBBXXBX、XBBXBX和ΨBΨXB以及单链和双链NLS序列在ApoE和ApoB100以及囊膜蛋白和衣壳蛋白中含量丰富。人工合成的DENV2包膜蛋白序列的荧光标记多肽以及包含这些基序的DENV3衣壳被用来定性地评估细胞结合和进入HeLa细胞的能力。DENV2包膜多肽Dsp2EP，0564Gly-Gly0595结合并保持在细胞表面。相比之下，DENV3衣壳蛋白多肽Dsp3CP，0002Asn-Gln0028很容易进入HeLa细胞，并在细胞核内的离散位置聚集。FITC标记的登革热合成肽与低密度脂蛋白-CM-DII和载脂蛋白E-CM-DII共定位到一定程度，表明登革病毒可能利用LLP所使用的细胞进入途径。
It is established that cell entry of low density lipoprotein particles (LLPs) containing Apo B100 and Apo E is mediated by receptors and GAGs. Receptor ligand motifs, XBBBXXBX, XBBXBX, and ΨBΨXB, and mono- and bipartite NLS sequences are abundant in Apo E and Apo B100 as well as in envelope and capsid proteins of Dengue viruses 1-4 (DENV1-4). Synthetic, fluorescence-labeled peptides of sequences in DENV2 envelope protein, and DENV3 capsid that include these motifs were used to conduct a qualitative assessment of cell binding and entry capacity using HeLa cells. DENV2 envelope peptide, Dsp2EP, 0564Gly-Gly0595, was shown to bind and remain at the cell surface. In contrast, DENV3 capsid protein peptide, Dsp3CP, 0002Asn-Gln0028, readily enters HeLa cells and accumulates at discrete loci in the nucleus. FITC-labeled dengue synthetic peptides colocalize with Low Density Lipoprotein-CM-DiI and Apo E-CM-DiI to a degree that suggests that Dengue viruses may utilize cell entry pathways used by LLPs.