Assembly factors for the membrane arm of human complex I
Assembly factors for the membrane arm of human complex I
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DOI：
10.1073/pnas.1319247110
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发表时间：
2013-11-19
影响因子：
11.1
通讯作者：
Walker, John E.
中科院分区：
文献类型：
--
作者：
Andrews, Byron;Carroll, Joe;Walker, John E.
线粒体呼吸复合物I是核基因组和线粒体基因组的产物。线粒体DNA中编码的7个亚基整合到内膜中，它们与14个核编码的膜亚基的结合，从23个额外的核编码蛋白质、铁硫簇和黄素单核苷酸辅因子构建外在臂需要组装因子的参与。有些是内在的复杂，而其他参与短暂。复合物I的NDUFA 11亚基的表达的抑制破坏了复合物的组装，并且积累了具有550和815 kDa的质量的亚复合物。八个已知的外在组装因子加上疏水蛋白，C3orf1，与亚复合物。C3orf 1、另一种组装因子TMEM 126 B和NDUFA 11的特征表明它们都参与构建复合物I的膜臂。
Mitochondrial respiratory complex I is a product of both the nuclear and mitochondrial genomes. The integration of seven subunits encoded in mitochondrial DNA into the inner membrane, their association with 14 nuclear-encoded membrane subunits, the construction of the extrinsic arm from 23 additional nuclear-encoded proteins, iron-sulfur clusters, and flavin mononucleotide cofactor require the participation of assembly factors. Some are intrinsic to the complex, whereas others participate transiently. The suppression of the expression of the NDUFA11 subunit of complex I disrupted the assembly of the complex, and subcomplexes with masses of 550 and 815 kDa accumulated. Eight of the known extrinsic assembly factors plus a hydrophobic protein, C3orf1, were associated with the subcomplexes. The characteristics of C3orf1, of another assembly factor, TMEM126B, and of NDUFA11 suggest that they all participate in constructing the membrane arm of complex I.