Effect of ovarian hormones on periodical changes in immune resistance associated with estrous cycle in the beagle bitch
Effect of ovarian hormones on periodical changes in immune resistance associated with estrous cycle in the beagle bitch
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DOI：
10.1016/j.imbio.2004.09.003
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发表时间：
2004-01-01
期刊：
影响因子：
2.8
通讯作者：
Inaba, T
中科院分区：
文献类型：
--
作者：
Sugiura, K;Nishikawa, M;Inaba, T
在母犬身上，子宫积脓是一种子宫感染，典型地发生在发情周期的间情期的前半部分，在这一阶段，孕酮的血液浓度最高，雌二醇-17β的血液浓度最低。为了探讨子宫积脓阶段特异性发病的免疫学机制，采集了发情周期不同阶段比格犬的外周血单核细胞(PBMNCs)，并用不同的免疫学方法进行了检测。当我们检测PYO-252(一种从患子宫积脓的狗的子宫中分离的大肠杆菌克隆)对PBMNCs的增殖反应时，PBMNCs对PYO-252的反应在发情前期(第10天)显著降低，而在发情前期/发情前期增加。在周期的不同阶段对刀豆素A的反应没有显著差异。在整个发情周期中，犬的PBMNCs对来自大肠杆菌的脂多糖没有反应。在发情期采集的PBMNCs对PYO-252的反应在培养中加入雌二醇-17β后显著增强。相反，在黄体酮的存在下，这些反应被显著抑制。孕激素前体或代谢物分子与孕激素受体的亲和力较低，不影响增殖反应。17β雌二醇显著增强PYO-252诱导的干扰素表达，而孕酮则抑制干扰素的表达。这一证据表明，在动情周期的前半段，细胞免疫活性受到抑制，原因是孕酮浓度增加和雌激素释放减少。免疫抵抗力的显著下降使大肠杆菌得以扩张，在发情期间通过松动的宫颈进入宫腔，导致子宫积脓。(C)2004年爱思唯尔股份有限公司。版权所有。
In bitches, the onset of pyometra, an infection of the uterus, characteristically occurs in the first half of the diestrous stage in the estrous cycle, in which the blood concentration of progesterone peaks and that of estradiol-17beta is lowest. To investigate the immunological mechanisms governing stage-specific onset of pyometra, peripheral blood mononuclear cells (PBMNCs) were collected from beagle bitches during different stages of the estrous cycle and examined using various immunological assays. When we examined the proliferative response of PBMNCs to PYO-252, that is a clone of Escherichia coli isolated from the uterus of a dog afflicted with pyometra, the response of PBMNCs significantly decreased in the first half (day 10) of diestrus, but increased in proestrus/estrus. No significant differences were observed in the responses to concanavaline A between stages of the cycle. Throughout the estrous cycle, canine PBMNCs did not respond to lipopolysaccharide derived from E coli. The response of PBMNCs collected in anestrus to PYO-252 was significantly enhanced upon the addition of estradiol-17beta to the culture. In contrast, these responses were significantly suppressed in the presence of progesterone. Progesterone progenitor or metabolite molecules, which have a low affinity for the progesterone receptor, did not affect proliferative responses. Expression of gamma interferon (IFN) in response to PYO-252 was also significantly enhanced by estradiol-17beta, but suppressed by progesterone. This evidence suggests that in the first half of the diestrous stage, suppressed activity of cellular immunity results from increasing progesterone concentration and minimal estrogen release. This marked decrease of immune resistance allows the expansion of E coli, which enter the uterine cavity through the loosened cervical canal during estrus, leading to pyometra onset. (C) 2004 Elsevier GmbH. All rights reserved.