Role of the isthmus and FGFs in resolving the paradox of neural crest plasticity and prepatterning
Role of the isthmus and FGFs in resolving the paradox of neural crest plasticity and prepatterning
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DOI：
10.1126/science.1064540
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发表时间：
2002-02-15
期刊：
影响因子：
56.9
通讯作者：
Krumlauf, R
中科院分区：
文献类型：
--
作者：
Trainor, PA;Ariza-McNaughton, L;Krumlauf, R
脑神经脊细胞在脊椎动物头部产生独特的骨和结缔组织。经典的颅面发育模型认为，神经脊在从神经管迁移之前是预先设计好的或预先编程的，以形成特定的头部结构。相比之下，最近对几种脊椎动物的研究为形成神经脊种群的可塑性提供了证据。通过在禽类胚胎中进行组织转位和分子分析，我们证明了构成前模式模型基础的经典操作实验涉及移植局部信号中心，即峡部组织者，从而协调了这些发现。来自峡部的Fgf8信号改变了Hoxa2的表达，从而改变了鳃。拱形图案，表明神经脊细胞是由环境信号图案形成的。
Cranial neural crest cells generate the distinctive bone and connective tissues in the vertebrate head. Classical models of craniofacial development argue that the neural crest is prepatterned or preprogrammed to make specific head structures before its migration from the neural tube. In contrast, recent studies in several vertebrates have provided evidence for plasticity in patterning neural crest populations. Using tissue transposition and molecular analyses in avian embryos, we reconcile these findings by demonstrating that classical manipulation experiments, which form the basis of the prepatterning model, involved transplantation of a local signaling center, the isthmic organizer. FGF8 signaling from the isthmus alters Hoxa2 expression and consequently branchial. arch patterning, demonstrating that neural crest cells are patterned by environmental signals.