Chromatin components as part of a putative transcriptional repressing complex
Chromatin components as part of a putative transcriptional repressing complex
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DOI：
10.1073/pnas.95.13.7322
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发表时间：
1998-06-23
影响因子：
11.1
通讯作者：
Ptashne, M
中科院分区：
文献类型：
--
作者：
Lehming, N;Le Saux, A;Ptashne, M
果蝇HMG 1样蛋白DSP1在酵母中以启动子特异性方式抑制Dorsal的转录激活功能。我们发现DSP1和HMG 2一样与哺乳动物蛋白SP 100 B结合，而SP 100 B又与人的HP 1蛋白结合，后者是一种参与转录沉默的果蝇蛋白。这些蛋白质中的每一种在与哺乳动物细胞中的DNA结合时都抑制转录。这些结果表明，异染色质蛋白可能被招募到DNA上的特定位点，从而对基因表达产生特定的影响。
The Drosophila HMG1-like protein DSP1 Has identified by its ability to inhibit the transcriptional activating function of Dorsal in a promoter-specific fashion in yeast, We show here that DSP1 as Hell as its mammalian homolog hHMG2 bind to the mammalian protein SP100B and that SP100B in turn binds to human homologs of HP1, The latter is a Drosophila protein that is inolved in transcriptional silencing. Each of these proteins represses transcription when tethered to DNA in mammalian cells. These results suggest how heterochromatin proteins might be recruited to specific sites on DNA with resultant specific effects on gene expression.