Semipermeable polymer vesicle (PICsome) self-assembled in aqueous medium from a pair of oppositely charged block copolymers: Physiologically stable micro-/nanocontainers of water-soluble macromolecules
Semipermeable polymer vesicle (PICsome) self-assembled in aqueous medium from a pair of oppositely charged block copolymers: Physiologically stable micro-/nanocontainers of water-soluble macromolecules
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DOI：
10.1021/ja057993r
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发表时间：
2006-05-10
影响因子：
15
通讯作者：
Kataoka, Kazunori
中科院分区：
文献类型：
--
作者：
Koide, Aya;Kishimura, Akihiro;Kataoka, Kazunori
通过将具有生物相容性的聚乙二醇（PEG）和聚氨基酸（PAn）组成的一对带相反电荷的嵌段共聚物在水介质中简单混合，制备了一种新型的具有聚离子复合物（PIC）膜的聚合物囊泡--PICsome。流动粒子图像分析显示形成了尺寸范围达10 μm的球形粒子。通过暗场和共聚焦激光扫描显微镜的观察清楚地证实了PIC体具有中空结构，具有内水相，其中发射绿色荧光的FITC-葡聚糖通过与嵌段共聚物同时混合而成功地包封。共聚焦激光扫描显微镜观察和光谱分析揭示了发射红色荧光的低分子量荧光染料（TRITC; MW = 443.5）顺利渗透到FITC-葡聚糖包封的PIC小体中，以给出具有合并的黄色的PIC小体图像，表明PIC小体膜的半渗透性质。PICsome显示出明显的生理稳定性，即使在血清蛋白的存在下，这表明它们在生物医学领域的可行性，如治疗化合物的载体和诊断酶的隔室。
A new entity of polymer vesicle with a polyion complex (PIC) membrane, a PICsome, was prepared by simple mixing of a pair of oppositely charged block copolymers, composed of biocompatible PEG and poly(amino acid)s, in an aqueous medium. Flow particle image analysis revealed the formation of spherical particles with a size range up to 10 μm. Observation by dark-field and confocal laser scanning microscopes clearly confirmed that the PICsome has a hollow structure with an inner-water phase, in which FITC-dextran emitting green fluorescence was successfully encapsulated simply by the simultaneous mixing with the block copolymers. Confocal laser scanning microscopic observation and spectral analysis revealed the smooth penetration of a low molecular weight fluorescent dye (TRITC; MW = 443.5) emitting red fluorescence into the FITC-dextran encapsulated PICsome to give the PICsome image with a merged color of yellows, indicating the semipermeable nature of the PICsome membrane. The PICsomes showed appreciable physiological stability even in the presence of serum proteins, suggesting their feasibility in biomedical fields such as carriers of therapeutic compounds and compartments for diagnostic enzymes.