NITRIC-OXIDE SYNTHASE - IRREVERSIBLE INHIBITION BY L-NG-NITROARGININE IN BRAIN INVITRO AND INVIVO
NITRIC-OXIDE SYNTHASE - IRREVERSIBLE INHIBITION BY L-NG-NITROARGININE IN BRAIN INVITRO AND INVIVO
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DOI：
10.1016/0006-291x(91)90403-t
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发表时间：
1991-05-15
影响因子：
3.1
通讯作者：
SNYDER, SH
中科院分区：
文献类型：
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作者：
DWYER, MA;BREDT, DS;SNYDER, SH
脑制剂中的 LNG-硝基精氨酸 (NO 2 Arg) 对一氧化氮 (NO) 合酶活性的抑制不会通过透析逆转，而是通过长时间预孵育 NO 2 Arg 与酶而增强。相比之下，NO 2 Arg 对巨噬细胞 NO 合酶的较弱抑制是完全可逆的。腹腔注射 5 或 50 mg/kg 后，NO 2 Arg 抑制大脑中的 NO 合酶活性。这种体内抑制似乎也是不可逆的。 NO 2 Arg 对中枢 NO 合酶的有效体内抑制可能有助于研究 NO 作为神经元信使的生理功能。
Inhibition of nitric oxide (NO) synthase activity by LN G-Nitroarginine (NO 2 Arg) in brain preparations is not reversed by dialysis and is enhanced by prolonged preincubation of NO 2 Arg with the enzyme. By contrast, the weaker inhibition by NO 2 Arg of macrophage NO synthase is fully reversible. NO 2 Arg inhibits NO synthase activity in the brain after ip administration of 5 or 50 mg/kg. This in vivo inhibition also appears to be irreversible. The potent in vivo inhibition of central NO synthase by NO 2 Arg may facilitate studies of the physiologic function of NO as a neuronal messenger.