The association of the activation-inducible tumor necrosis factor receptor and ligand with lumbar disc herniation
The association of the activation-inducible tumor necrosis factor receptor and ligand with lumbar disc herniation
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DOI：
10.3349/ymj.2007.48.5.839
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发表时间：
2007-10-31
影响因子：
2.4
通讯作者：
Riew, K. Daniel
中科院分区：
文献类型：
--
作者：
Park, Moon-Soo;Lee, Hwan-Mo;Riew, K. Daniel
目的：髓核突出碎片被免疫系统识别为异物，导致自身免疫反应。人活化诱导的肿瘤坏死因子受体(AITR)及其配体AITRL是自身免疫性疾病发病机制中重要的共刺激分子。尽管这些共刺激分子在自身免疫性疾病中很重要，但它们在对突出的椎间盘碎片的自身免疫反应中的作用仍有待研究。本研究的目的是探讨AITR和AITRL的过度表达是否可能与腰椎间盘突出症有关。材料和方法：研究人群包括20名有症状的腰椎间盘突出症患者。10个宏观上正常的对照椎间盘来自脊柱骨折患者，这些患者接受了前路手术，其中包括椎间盘切除。采集了研究患者和对照组的外周血样。用流式细胞仪、激光共聚焦扫描显微镜、免疫组织化学和逆转录聚合酶链式反应(RT-PCR)检测AITR和AITRL的表达水平。用双抗体夹心法测定血清中可溶性AITR和AITRL水平。结果：流式细胞仪分析显示腰椎间盘突出症患者的AITR和AITRL水平均显著高于对照组。激光共聚焦扫描显微镜、免疫组织化学和RT-PCR显示，AITRL在腰椎间盘突出症患者中的表达水平也增加。最后，腰椎间盘突出症患者的可溶性AITR和AITRL升高。结论：腰椎间盘突出症患者腰椎间盘组织和外周血中ATTR值和AITRL值均升高。
Purpose: Herniated nucleus pulposus fragments are recognized by the immune system as a foreign-body, which results in an autoimmune reaction. Human activation-inducible tumor necrosis factor receptor (AITR) and its ligand, AITRL, are important costimulatory molecules in the pathogenesis of autoimmune diseases. Despite the importance of these costimulatory molecules in autoimmune disease, their role in the autoimmune reaction to herniated disc fragments has yet to be explored. The purpose of the present study is to investigate whether the overexpression of AITR and AITRL might be associated with lumbar disc herniation. Materials and Methods: The study population consisted of 20 symptomatic lumbar disc herniation patients. Ten macroscopically normal control discs were obtained from patients with spinal fractures managed with anterior procedures that involved a discectomy. Peripheral blood samples from both the study patients and controls were collected. The expression levels of AITR and AITRL were investigated by flow cytometric analysis, confocal laser scanning microscopy, immunohistochemistry and by reverse transcriptase-polymerase chain reaction (RT-PCR). The soluble AITR and AITRL serum levels were measured by an enzyme-linked immunosorbent assay. Results: Flow cytometric analysis revealed significantly higher levels of both AITR and AITRL in the lumbar disc herniation patients than in the controls. The AITRL expression levels were also increased in patients with lumbar disc herniation, shown by using confocal laser scanning microscopy, immunohistochemistry, and RT-PCR. Finally, soluble AITR and AITRL were elevated in the patients with lumbar disc herniations. Conclusion: The ATTR and AITRL are increased in both the herniated disc tissue and the peripheral blood of patients with lumbar disc herniation.