Preparation, Characterization, and Antiproliferative Activities of the Se-Containing Polysaccharide SeGLP-2B-1 from Se-Enriched Ganoderma lucidum
Preparation, Characterization, and Antiproliferative Activities of the Se-Containing Polysaccharide SeGLP-2B-1 from Se-Enriched Ganoderma lucidum
复制标题
富硒灵芝含硒多糖SeGLP-2B-1的制备、表征及其抗增殖活性
DOI：
10.1021/jf9019344
复制
发表时间：
2009-09-09
影响因子：
6.1
通讯作者：
Cui, Qiao
中科院分区：
文献类型：
--
作者：
Shang, Dejing;Zhang, Jianing;Cui, Qiao
Inorganic selenite can be transformed into organic forms and bind to proteins and polysaccharides in Se-enriched submerged Ganoderma lucidum cultures. In the present study, a novel Se-containing polysaccharide, SeGLP-2B-1, was purified from the Se-enriched mycelia of G. lucidum and the antiproliferative activities against six human cancer cell lines were investigated. The Se content of SeGLP-2B-1 was 186.7 mu g/g, which was 150-fold larger than that of the regular polysaccharide GLP-2B-1 (1.31 mu g/g). SeGLP-2B-1 (1.06 x 10(8) Da) was composed of glucose, rhamnose, xylose, and galactose with a molar ratio of 1.000:0.652:0.443:0.227. SeGLP-2B-1 exhibited an approximately 10-fold stronger antiproliferative activity against six human cancer cell lines as compared to GLP-2B-1. Thus, Se is believed to play an important role in increasing the antiproliferative property of SeGLP-2B-1. These findings indicate that SeGLP-2B-1 may serve as a dietary Se supplement.
