CD15 immunoreactive amacrine cells in the mouse retina
CD15 immunoreactive amacrine cells in the mouse retina
复制标题
小鼠视网膜中 CD15 免疫反应性无长突细胞
DOI：
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发表时间：
2003
期刊：
影响因子：
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通讯作者：
R. Masland
中科院分区：
文献类型：
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作者：
T. Jakobs;Y. Ben;R. Masland
小鼠视网膜已成为视觉研究的重要模型，主要是因为转基因动物的广泛应用。为了研究视网膜内部的细胞功能和连通性，对一种细胞类型或少数细胞类型进行不同染色的抗体作为标记是有帮助的。在这里，我们使用免疫荧光共聚焦显微镜和逆转录聚合酶链式反应来鉴定小鼠视网膜中的CD15(3[α1‐3]‐fucosyl‐N‐acetyl‐lactosamine)‐positive细胞。CD15免疫反应见于两种不同类型的无长突细胞和少量锥体双极细胞。I型CD15+无长突细胞是GABA能的广域细胞，分布于内网状层的第3层和第4/5层。II型CD15+无长突细胞也是GABA能细胞，并与内丛状层1层中的多巴胺能酪氨酸羟基酶阳性细胞共存。中周I型和II型CD15+无长突细胞密度分别为258个/mm2和274个/mm2。与其他几种无长突细胞类型的标记双重标记表明，这两种类型都不属于先前发现的另一种无长突细胞亚群。单细胞RT-PCR显示CD15+无长突细胞共表达几种AMPA受体--GluR1、GluR2和GluR4是最常见的组合。J.Comp.神经。465：361-371,2003。©2003 Wiley-Liss公司
The mouse retina has become an important model in vision research, mainly because of the wide availability of transgenic animals. In order to study cell function and connectivity in the inner retina, antibodies that differentially stain one cell type, or a small number of cell types, are helpful as markers. Here we characterize the CD15 (3[α1‐3]‐fucosyl‐N‐acetyl‐lactosamine)‐positive cells in the mouse retina using immunofluorescence confocal microscopy and reverse‐transcription polymerase chain reaction. CD15 immunoreactivity was observed in two distinct types of amacrine cells and, faintly, in some cone bipolar cells. Type I CD15+ amacrine cells are GABAergic wide‐field cells that stratify in lamina 3 and 4/5 of the inner plexiform layer. Type II CD15+ amacrine cells are also GABAergic and costratify with the dopaminergic tyrosine hydroxylase‐positive cells in lamina 1 of the inner plexiform layer. The densities of types I and II CD15+ amacrine cells in mid‐periphery were 258 cells/mm2 and 274 cells/mm2. Double labeling with several other markers for amacrine cell types showed that neither type belongs to another previously identified subpopulation of amacrine cells. Single‐cell RT‐PCR showed that CD15+ amacrine cells coexpress several AMPA receptors — GluR1, GluR2, and GluR4 being the most common combination. J. Comp. Neurol. 465:361–371, 2003. © 2003 Wiley‐Liss, Inc.