The rice pyruvate decarboxylase 3 gene, which lacks introns, is transcribed in mature pollen

The rice pyruvate decarboxylase 3 gene, which lacks introns, is transcribed in mature pollen
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DOI:
10.1093/jxb/erh029
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发表时间:
2004-01-01
影响因子:
6.9
通讯作者:
Nakazono, M
Nakazono, M
中科院分区:
生物学1区
文献类型:
--
作者:
Li, YH;Ohtsu, K;Nakazono, M

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水稻丙酮酸脱羧酶3基因(rice pyruvate decarboxylase 3 gene, PDC3)不含内含子,以前被认为是假基因,因为即使在厌氧条件下也没有检测到PDC3 mRNA。但本研究发现,水稻抽穗后,PDC3转录本在穗内积累。在花药中,通过原位杂交发现PDC3在成熟花粉中转录。这些结果表明,水稻PDC3是一个功能基因。其产物可能在成熟花粉的有氧酒精发酵中起作用。
The rice pyruvate decarboxylase 3 gene (PDC3), which has no introns, was previously postulated to be a pseudogene because no PDC3 mRNA had been detected, even under anaerobic conditions. However, in this study, it was found that rice PDC3 transcripts accumulated in panicles after heading. Within anthers obtained from the panicles, PDC3 was shown to be transcribed in mature pollen by in situ hybridization. These results suggest that the rice PDC3 is a functional gene. Its product may play a role in aerobic alcoholic fermentation in mature pollen.