MicroRNA expression in the adult mouse central nervous system
MicroRNA expression in the adult mouse central nervous system
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DOI：
10.1261/rna.783108
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发表时间：
2008-03-01
期刊：
影响因子：
4.5
通讯作者：
Kauppinen, Sakari
中科院分区：
文献类型：
--
作者：
Bak, Mads;Silahtaroglu, Asli;Kauppinen, Sakari
MicroRNAs是一种22个核苷酸的内源性非编码RNA，它通过与靶mRNAs的39个非翻译区进行碱基配对，在转录后抑制蛋白质编码基因的表达。我们在这里提供了成年小鼠中枢神经系统(CNS)13个神经解剖学不同区域的miRNA表达谱清单。结合实时RT-PCR和基于LNA(锁定核酸)的原位杂交，微阵列分析发现44个miRNAs在脊髓、小脑、延髓、脑桥、下丘脑、海马体、新皮质、嗅球、眼睛和脑下垂体中表达超过三倍。这些发现表明，大量小鼠中枢神经系统表达的miRNAs可能与这些区域内的特定功能有关。值得注意的是，尽管成熟的miRNA序列在两种脊椎动物之间几乎100%保守，但超过50%已鉴定的小鼠中枢神经系统富含miRNAs的miRNAs显示出与已报道的斑马鱼不同的表达模式。本文提供的成年小鼠中枢神经系统miRNA图谱的清单为进一步阐明哺乳动物中枢神经系统中miRNA的功能和miRNA相关的基因调控网络提供了重要的一步。
microRNAs are; 22 nucleotide endogenous noncoding RNAs that post-transcriptionally repress expression of protein-coding genes by base-pairing with the 39-untranslated regions of the target mRNAs. We present here an inventory of miRNA expression profiles from 13 neuroanatomically distinct areas of the adult mouse central nervous system (CNS). Microarray profiling in combination with real-time RT-PCR and LNA (locked nucleic acid)-based in situ hybridization uncovered 44 miRNAs displaying more than threefold enrichment in the spinal cord, cerebellum, medulla oblongata, pons, hypothalamus, hippocampus, neocortex, olfactory bulb, eye, and pituitary gland. These findings suggest that a large number of mouse CNS-expressed miRNAs may be associated with specific functions within these regions. Notably, more than 50% of the identified mouse CNS-enriched miRNAs showed different expression patterns compared to those reported in zebrafish, although the mature miRNA sequences are nearly 100% conserved between the two vertebrate species. The inventory of miRNA profiles in the adult mouse CNS presented here provides an important step toward further elucidation of miRNA function and miRNA-related gene regulatory networks in the mammalian central nervous system.