The global phylogeny of glycolytic enzymes
The global phylogeny of glycolytic enzymes
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DOI：
10.1073/pnas.082112499
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发表时间：
2002-04-30
影响因子：
11.1
通讯作者：
Kurland, CG
中科院分区：
文献类型：
--
作者：
Canback, B;Andersson, SGE;Kurland, CG
编码兼性胞内寄生虫汉赛巴尔通体（Bartonella henselae）糖酵解酶的基因已在来自细菌和真核生物的大量同源物中进行了遗传学分析。我们专注于这个相对的普氏立克次氏体沿着与同源物从其他α-变形菌，以确定是否有系统的糖酵解基因转移从假定的α-变形菌的祖先的古细菌的细胞核的早期真核生物。代表此处研究的八种糖酵解酶的α-蛋白细菌同源物往往聚集在支持良好的节点中。然而，这些α-变形菌的酶中没有一个与相应的真核同源物作为姐妹进化枝相关。真核生物的糖酵解基因与其他细菌门的糖酵解基因之间也没有密切的系统发育关系。相反，几个重建表明，可能有系统的转移编码糖酵解酶的序列从蓝藻到一些绿色植物。否则，令人惊讶地观察到细菌和真核结构域之间的交换很少。真核生物中间代谢酶编码基因的下降被重新评估。
Genes encoding the glycolytic enzymes of the facultative endocellular parasite Bartonella henselae have been analyzed phylogenetically within a very large cohort of homologues from bacteria and eukaryotes. We focus on this relative of Rickettsia prowazekii along with homologues from other alpha-proteobacteria to determine whether there have been systematic transfers of glycolytic genes from the presumed alpha-proteobacterial ancestor of the mitochondrion to the nucleus of the early eukaryote. The alpha-proteobacterial homologues representing the eight glycolytic enzymes studied here tend to cluster in well-supported nodes. Nevertheless, not one of these alpha-proteobacterial enzymes is related as a sister clade to the corresponding eukaryotic homologues. Nor is there a close phylogenetic relationship between glycolytic genes from Eucarya and any other bacterial phylum. In contrast, several of the reconstructions suggest that there may have been systematic transfer of sequences encoding glycolytic enzymes from cyanobacteria to some green plants. Otherwise, surprisingly little exchange between the bacterial and eukaryotic domains is observed. The descent of eukaryotic genes encoding enzymes of intermediary metabolism is reevaluated.