A specific "switch-on" type magnetic resonance nanoprobe with distance-dominate property for high-resolution imaging of tumors
A specific "switch-on" type magnetic resonance nanoprobe with distance-dominate property for high-resolution imaging of tumors
复制标题
一种具有距离主导特性的特定“开启”型磁共振纳米探针，用于肿瘤高分辨率成像
DOI：
10.1016/j.cej.2020.126496
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发表时间：
2021-01-15
影响因子：
15.1
通讯作者：
Shi, Jinjin
中科院分区：
文献类型：
--
作者：
Liu, Junjie;Yu, Wenyan;Shi, Jinjin
磁共振成像（MRI）造影剂（CA）是疾病诊断的有力工具。然而，临床上使用的CA的“始终开启”状态影响体内肿瘤MR成像的特异性。在此，我们构建了一个肿瘤微环境的“开关”型纳米探针（TSN）的距离占主导地位的特性，为特定的肿瘤MR成像，采用超顺磁性中空介孔氧化铁纳米粒子（HMIN）作为载体，以封装T-1场CA（Gd），然后与生物相容性的透明质酸（HA）的介孔通道的封闭。在低水平透明质酸酶（HAase）的正常组织中，Gd被限制在HMIN内部，两种物质之间的距离变得相当接近，Gd的磁场受到超顺磁性HMIN的干扰，关闭T-1场信号;相反，在具有高水平HAase的肿瘤组织中，Gd通过特异性响应于过表达的HAase而从HMIN快速释放，两种物质之间的距离显著增大，Gd的磁场不受干扰，从而“开启”T-1场信号，以距离受控的方式实现肿瘤特异性“开启”MR成像。TSN治疗后的肿瘤/正常T-1场信号比是游离Gd-DTPA的1.9倍，这是由于其高Gd载量、HA的肿瘤靶向性和肿瘤特异性。这种距离控制的可切换纳米探针提供了一种具有高特异性的高分辨率肿瘤成像的替代策略。
Magnetic resonance imaging (MRI) contrast agents (CAs) are a powerful tool for disease diagnosis. However, the "always on" state of clinically used CAs affects the specificity of tumor MR imaging in vivo. Herein, we constructed a tumor microenvironment "switch-on" type nanoprobe (TSN) with a distance-dominated property for specific tumor MR imaging, by employed a superparamagnetic hollow mesoporous iron oxide nanoparticle (HMIN) as the carrier to encapsulate T-1-field CA (Gd), followed by blocking of the mesoporous channel with biocompatible hyaluronic acid (HA). In normal tissues with low-level hyaluronidase (HAase), Gd was restricted inside the HMIN, the distance between the two substances became quite close, and the magnetic field of Gd was interfered by the superparamagnetic HMIN, turning "off" the T-1-field signal is "turned off"; in contrast, in tumor tissues with high-level HAase, Gd was rapidly released from the HMIN by specifically responding to over-expressed HAase, the distance between the two substances became significantly enlarged, and the magnetic field of Gd was not interfered, thus turning "on" the T-1-field signal to achieve tumor-specific "switch on" MR imaging in a distance-controlled manner. The tumor/normal T-1-field signal ratio after TSN treatment increased by 1.9-fold compared with free Gd-DTPA, because of its high Gd loading content, tumor-targeting ability of HA and the tumor-specific property. This distance-controlled switchable nanoprobe provides an alternative strategy for high-resolution tumor imaging with high specificity.