DESI-MS imaging of lipids and metabolites from biological samples.
DESI-MS imaging of lipids and metabolites from biological samples.
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DOI：
10.1007/978-1-4939-1258-2_20
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发表时间：
2014-01-01
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影响因子：
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通讯作者：
Eberlin, Livia S
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作者：
Eberlin, Livia S
解吸电喷雾离子化质谱（ESIMS）是一种强大的技术，用于直接从生物样品中成像数百个小分子的分布，而不需要大量的样品制备。在环境温度和露天环境下操作，EST-MS采用带电液滴的精细喷雾，将分子从样品表面快速提取到溶剂液滴中，并将分析物转移到质谱仪。当在成像模式下操作时，样品表面内的化合物的二维图是以数百微米或更小的典型空间分辨率获得的。在这里，我们描述了使用组织学相容的溶剂系统从小鼠脑组织切片的复杂脂质的成像。所描述的成像实验使用用于质量分析的Orbitrap质谱仪在负离子模式下进行。在EST-MS成像后，对相同的组织切片进行组织化学，使分子信息与组织学信息相关联。
Desorption electrospray ionization mass spectrometry (DESI-MS) is a powerful technique used to image the distribution of hundreds of small molecules directly from biological samples, without the need of extensive sample preparation. Operated at ambient temperature and in the open-air environment, DESI-MS employs a fine spray of charged droplets to rapidly extract molecules from the sample surface into the solvent droplets and to transfer the analytes to the mass spectrometer. When operated in the imaging mode, a two-dimensional map of the compounds within the sample surface is obtained with a typical spatial resolution of hundreds of micrometers or less. Here, we describe the imaging of complex lipids from a mouse brain tissue section using a histologically compatible solvent system. The imaging experiment described is performed in the negative ion mode using an Orbitrap mass spectrometer for mass analysis. Following DESI-MS imaging, the same tissue section is subjected to histochemistry allowing molecular information to be correlated to histological information.