Chemical-Hydrothermal Synthesis of Bioinert ZrO2-TiO2 Films on Pure Ti Substrates and Proliferation of Osteoblast-Like Cells
Chemical-Hydrothermal Synthesis of Bioinert ZrO2-TiO2 Films on Pure Ti Substrates and Proliferation of Osteoblast-Like Cells
复制标题
纯钛基底上生物惰性 ZrO2-TiO2 薄膜的化学水热合成及成骨细胞样细胞的增殖
DOI：
--
复制
发表时间：
2009
期刊：
影响因子：
--
通讯作者：
T. Nakano
中科院分区：
文献类型：
--
作者：
M. Ueda;Y. Sasaki;M. Ikeda;M. Ogawa;W. Fujitani;T. Nakano
研究了用化学水热法在纯钛表面合成生物惰性氧化膜。在水热处理之前，用H2O2/HNO3对一些纯Ti衬底进行化学处理，形成TiO2凝胶层。水热处理制备了两种溶液：Zr(OH)4凝胶分散NH3/CH3CH(OH)COOH溶液和Zr溶解NH3/CH3CH(OH)COOH溶液。经水热处理的试样表面形成了一层均匀的极细晶体。水热处理后出现了TiO2和/或ZrO2的尖峰。用Zr(OH)4凝胶分散的NH3/CH3CH(OH)COOH溶液在453k下水热处理12 h，在Ti表面成功制备了ZrO2-TiO2复合薄膜。我们还研究了成骨细胞样MC3T3-E1细胞的增殖情况。观察到细胞的假足扩散。细胞密度随孵育时间的延长而单调增加。细胞的增殖受表面粗糙度的影响较大；在Ra¼20{80 nm范围内，细胞密度随粗糙度的减小而增大。此外，表面产物TiO2的增殖率相对较低。细胞与表面的强烈附着可能阻碍细胞迁移。(doi: 10.2320 / matertrans.MAW200910)
The synthesis of bioinert oxide films on pure Ti surfaces by chemical-hydrothermal treatment was investigated. Some pure Ti substrates were chemically treated with H2O2/HNO3 to form a TiO2 gel layer prior to hydrothermal treatment. Two types of solutions in the hydrothermal treatment were prepared: Zr(OH)4 gel dispersed NH3/CH3CH(OH)COOH solution and Zr dissolved NH3/CH3CH(OH)COOH solution. A uniform layer of very fine crystals were formed on the surfaces of the specimens subjected to the hydrothermal treatment. Sharp peaks attributed to TiO2 and/or ZrO2 appeared after the hydrothermal treatment. ZrO2-TiO2 composite films were successfully produced on the Ti surface by hydrothermal treatment with the Zr(OH)4 gel dispersed NH3/CH3CH(OH)COOH solution at 453 K for 12 h, following the chemical treatment. The proliferation of osteoblast-like MC3T3-E1 cells was also investigated. The cells were observed to have spread their pseudopodiums. The cell density increased monotonically with incubation period for all specimens. The proliferation of cells was strongly influenced by the surface roughness; the cell densities increased with decreasing roughness in the range of Ra ¼ 20{80 nm. Furthermore, the surface products with TiO2 showed a relatively low rate of proliferation. The strong attachment of cells to the surface may hinder cell migration. [doi:10.2320/matertrans.MAW200910]
影响因子：
14
作者：
Washburn, NR;Yamada, KM;Amis, EJ
通讯作者：
Amis, EJ