A DREB-Like Transcription Factor From Maize (Zea mays), ZmDREB4.1, Plays a Negative Role in Plant Growth and Development.
A DREB-Like Transcription Factor From Maize (Zea mays), ZmDREB4.1, Plays a Negative Role in Plant Growth and Development.
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来自玉米 (Zea mays) 的 DREB 样转录因子 ZmDREB4.1 在植物生长和发育中发挥负面作用
DOI：
10.3389/fpls.2018.00395
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发表时间：
2018
影响因子：
5.6
通讯作者：
Yu J
中科院分区：
文献类型：
--
作者：
Li S;Zhao Q;Zhu D;Yu J
DREB（脱水反应元件结合）型转录因子分为六个亚组，命名为A-1至A-6。DREB A-1和A-2亚组的成员已被报道参与响应各种非生物胁迫。然而，只有少数属于A-3至A-6亚组的基因被报道。本研究克隆了玉米DREB A-4亚类基因ZmDREB 4.1，并分析了其特性和功能。ZmDREB4.1在根、茎和叶中以非常低的水平表达。它不是由任何生物或非生物处理引起的。ZmDREB4.1定位于细胞核内，可直接与DRE元件结合，起转录激活因子的作用。ZmDREB4.1在烟草中的组成型表达抑制叶的伸长和下胚轴、叶柄和茎的伸长。在玉米中，ZmDREB4.1的过表达抑制愈伤组织的生长和再生。进一步分析表明，转基因烟草叶片变小是由于细胞分裂受到抑制。转基因植株叶片中细胞分裂素和生长素含量严重下降。转基因烟草的下胚轴、茎和叶柄变短是由于细胞伸长受到抑制所致。转基因下胚轴、茎和叶柄含有降低的赤霉素水平。外源GA 3处理能挽救较短的下胚轴、茎和叶柄，但不能挽救较小的叶片。这些结果表明ZmDREB4.1在植物生长发育的负调控中起重要作用。
The DREB (dehydration-responsive element binding)-type transcription factors are classified into six subgroups, named A-1 to A-6. The members of DREB A-1 and A-2 subgroups have been reported to be involved in response to various abiotic stresses. However, there were only a few genes belonging to A-3 to A-6 subgroups to be reported. In this study, we cloned a DREB A-4 subgroup gene from maize (Zea mays), ZmDREB4.1, and analyzed its characteristics and functions. ZmDREB4.1 was expressed in roots, stems, and leaves at very low levels. It was not induced by any biotic or abiotic treatment. ZmDREB4.1 was located in the nucleus, could directly bind to the DRE element and functioned as a transcriptional activator. The constitutive expression of ZmDREB4.1 in tobacco (Nicotiana tabacum L.) repressed leaf extension and hypocotyl, petiole and stem elongation. In maize, overexpression of ZmDREB4.1 repressed calli growth and regeneration. Further analysis showed that the smaller leaves of transgenic tobacco resulted from inhibition of cell division. The contents of cytokinin and auxin in transgenic leaves were severely decreased. The shorter hypocotyls, stems and petioles of transgenic tobacco were caused by inhibition of cell elongation. The transgenic hypocotyls, stems and petioles contained reduced gibberellin levels. Application of exogenous GA3 rescued the shorter hypocotyls, stems and petioles, but not the smaller leaves. These results demonstrated that ZmDREB4.1 plays an important role in the negative regulation of plant growth and development.
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影响因子：
11.6
作者：
Griffiths, Jayne;Murase, Kohji;Thomas, Stephen G.
通讯作者：
Thomas, Stephen G.
影响因子：
7.4
作者：
Gilmour, SJ;Sebolt, AM;Thomashow, MF
通讯作者：
Thomashow, MF
影响因子：
7.2
作者：
Kizis, D;Pagès, M
通讯作者：
Pagès, M
影响因子：
7.3
作者：
Huang, Jin-Guang;Yang, Mei;Zheng, Cheng-Chao
通讯作者：
Zheng, Cheng-Chao
影响因子：
11.6
作者：
JOFUKU, KD;DENBOER, BGW;OKAMURO, JK
通讯作者：
OKAMURO, JK