The study of anti-metabolic syndrome effect of puerarin in vitro
The study of anti-metabolic syndrome effect of puerarin in vitro
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DOI：
10.1016/j.lfs.2005.03.036
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发表时间：
2005-11-04
期刊：
影响因子：
6.1
通讯作者：
Chen, G
中科院分区：
文献类型：
--
作者：
Xu, ME;Xiao, SZ;Chen, G
葛根素是从中国植物葛根中提取的一种异黄酮类化合物。奥维。据报道，它在治疗糖尿病和心血管疾病方面具有广泛的药理作用。本研究的目的是将葛根素的分散作用联系起来，并寻找其共同的作用机制。我们研究了葛根素对代谢综合征的关键常见致病因素的影响；这些因素包括肥胖、II型糖尿病和心血管疾病。最近，大量证据表明，胰岛素抵抗、脂肪细胞和内皮功能障碍之间存在复杂的相互作用，这些因素与代谢综合征的异常有关。研究结果表明，葛根素可促进胰岛素诱导的前脂肪细胞分化，促进高糖诱导的胰岛素抵抗脂肪细胞的葡萄糖摄取，防止肿瘤坏死因子-α诱导的内皮细胞凋亡和活力丧失。此外，我们发现这些作用可能是由于促进了PPARγ的表达，部分是通过抑制TNT-a诱导的内皮细胞内异常的游离钙积聚。总之，我们的综合研究将葛根素的综合药理作用与公认的代谢综合征的常见发病机制联系起来，为葛根素的作用机制提供了新的见解。(C)2005 Elsevier Inc.保留所有权利。
Puerarin is an isoflavone extracted from Chinese plant, Pueraria lobata (Wild.) Ohwi. It has been reported to have comprehensive pharmacological action in treatment of diabetes and cardiovascular diseases. The purpose of this study was to link the scattered effects of puerarin and to find the common mechanisms underlying. We investigated the effect of puerarin on the pivotal common pathogenic factors of metabolic syndrome; which includes obesity, Type II diabetes and cardiovascular diseases. Recently, a large body of evidence indicates that there is a complicated interplay among insulin resistance, adipocytes and endothelial dysfunction that links the abnormalities of metabolic syndrome. Results of present study showed that puerarin could potentiate insulin-induced preadipocyte differentiation, promote glucose-uptake of adipocytes that have been induced insulin resistance by high glucose, and prevent TNF-a-induced apoptosis and viability loss of endothelial cells. Furthermore, we found that these effects are probably due to promote PPAR gamma expression and partly through inhibiting abnormal TNT-a-induced intracellular-free Ca2+ accumulation of endothelial cells. Overall, our synthetical study links the comprehensive pharmacological actions of puerarin to the recognized common pathogenesis of metabolic syndrome, and provides a new insight into the mechanism of puerarin effect. (c) 2005 Elsevier Inc. All rights reserved.