Transcriptome profiling of the response of Arabidopsis suspension culture cells to Suc starvation
Transcriptome profiling of the response of Arabidopsis suspension culture cells to Suc starvation
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DOI：
10.1104/pp.104.044362
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发表时间：
2004-08-01
期刊：
影响因子：
7.4
通讯作者：
Bassham, DC
中科院分区：
文献类型：
--
作者：
Contento, AL;Kim, SJ;Bassham, DC
当遇到营养胁迫条件时，植物细胞会经历广泛的新陈代谢变化，并诱导营养循环途径以维持其持续生存。研究了营养物质动员在拟南芥悬浮细胞对蔗糖饥饿反应中的作用。空泡自噬在饥饿后24小时内被诱导，空泡蛋白水解酶的表达增加，这可能是提供给液泡的细胞质成分降解所必需的，从而实现营养循环。饥饿48h后，细胞活力开始下降，72h时细胞大量死亡。为了进一步了解在Suc缺乏期间生存所需的途径，使用包含22,810个探针集的ATH1基因芯片阵列进行了Suc饥饿期间的转录谱分析。在饥饿的48小时内，343个基因的转录水平显著增加，表明对营养胁迫的反应，利用细胞成分的循环和营养物质的清除来维持细胞功能，通过激活各种防御和应激反应途径来保护细胞免于死亡，以及通过特定的蛋白激酶和转录因子调节这些过程。这些生理和分子数据支持这样一个模型，即植物细胞在蔗糖耗尽时启动营养动员的协调反应，能够维持细胞活力长达48小时。在这一点之后，可能参与细胞死亡的基因表达增加，而那些参与翻译和复制的基因减少，导致培养活性和细胞死亡程序的激活。
Upon encountering nutrient stress conditions, plant cells undergo extensive metabolic changes and induce nutrient recycling pathways for their continued survival. The role of nutrient mobilization in the response of Arabidopsis suspension cells to Suc starvation was examined. Vacuolar autophagy was induced within 24 h of starvation, with increased expression of vacuolar proteases that are likely to be required for degradation of cytoplasmic components delivered to the vacuole, and thus for nutrient recycling. After 48 h of starvation, culture viability began to decrease, and substantial cell death was evident by 72 h. To provide further insight into the pathways required for survival during Suc deficit, transcriptional profiling during Suc starvation was performed using the ATH1 GeneChip array containing 22,810 probe sets. A significant increase in transcript levels was observed for 343 genes within 48 h of starvation, indicating a response to nutrient stress that utilizes the recycling of cellular components and nutrient scavenging for maintaining cell function, the protection of the cell from death through activation of various defense and stress response pathways, and regulation of these processes by specific protein kinases and transcription factors. These physiological and molecular data support a model in which plant cells initiate a coordinated response of nutrient mobilization at the onset of Suc depletion that is able to maintain cell viability for up to 48 h. After this point, genes potentially involved in cell death increase in expression, whereas those functioning in translation and replication decrease, leading to a decrease in culture viability and activation of cell death programs.