Activated Lymphocytes Secretome Inhibits Differentiation and Induces Proliferation of C2C12 Myoblasts
Activated Lymphocytes Secretome Inhibits Differentiation and Induces Proliferation of C2C12 Myoblasts
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DOI：
10.1159/000356655
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发表时间：
2014-01-01
影响因子：
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通讯作者：
Stewart, Claire E.
中科院分区：
文献类型：
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作者：
Al-Shanti, Nasser;Durcan, Peter;Stewart, Claire E.
背景/目标：衰老与免疫功能的显著下降有关，这可能有助于影响骨骼肌细胞再生过程的局部环境。研究方法：在此，我们专注于确定激活的免疫系统分泌组对成肌细胞分化和增殖的影响，作为减轻肌肉老化不良影响的可能手段。使用C2 C12成肌细胞作为模型以评估抗CD 3/IL-2活化后淋巴细胞条件培养基（CM）的影响。结果：与活化的淋巴细胞CM一起培养的成肌细胞表现出减少的形态和生化分化（98 ± 20，p
Background/Aims: ageing is associated with a marked decline in immune function which may contribute to the local environment that can influence the regenerative process of skeletal muscle cells. Methods: Herein, we focused on determining the effect of an activated immune system secretome on myoblast differentiation and proliferation as possible means to attenuate adverse effects of muscle aging. C2C12 myoblasts were used as model to assess the impact of lymphocyte conditioned media (CM) following anti-CD3/IL-2 activation. Results: Myoblasts cultured with activated lymphocytes CM exhibited reduced morphological and biochemical differentiation (98 +/- 20, p