Human platelet glycoproteins V and IX: mapping of two leucine-rich glycoprotein genes to chromosome 3 and analysis of structures.
Human platelet glycoproteins V and IX: mapping of two leucine-rich glycoprotein genes to chromosome 3 and analysis of structures.
复制标题
人血小板糖蛋白 V 和 IX：将两个富含亮氨酸的糖蛋白基因定位到 3 号染色体并进行结构分析。
DOI：
10.1021/bi00049a028
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发表时间：
1995
期刊：
影响因子：
2.9
通讯作者：
Roth,GJ
中科院分区：
文献类型：
--
作者：
Yagi,M;Edelhoff,S;Disteche,CM;Roth,GJ
材料和方法 GPV 和 GPIX 基因的分离和表征。先前已经描述了含有 GPV 基因的 X 噬菌体克隆的分离（Hickey 等，1993）。对于原位杂交实验，用GPV和GPIX基因特异的探针依次筛选人粘粒文库（Stratagene，WI38）。 GPV 探针用于筛选 X 文库，而 GPIX 特异性探针是从 GPIX 启动子区域分离的 Hindll-SacI 片段（GPV，Hickey 等，1993；GPIX，Hickey & Roth，1993）。两种探针均通过随机引发用 [a-32P] dCTP 进行标记。 GPV粘粒的身份通过限制性图谱和与特定寡核苷酸的杂交来确认，而GPIX粘粒的身份通过粘粒DNA的直接测序来验证。测序反应使用
MATERIALS AND METHODSIsolation and Characterization of the GPV and GPIX Genes. The isolation of the X phage clonecontaining the GPV gene has been describedpreviously (Hickey et al., 1993). For in situ hybridization experiments, a human cosmid library (Stratagene, WI38) was sequentially screened with probes specific for the GPV and GPIX genes. The GPV probe was that used to screen the X library, while the GPIX-specific probe was a Hindll\—Sacl fragmentisolated from the promoter region of GPIX (GPV, Hickey et al., 1993; GPIX, Hickey & Roth, 1993). Both probes were labeled with [a-32P] dCTP by random priming. The identityof the GPV cosmid was confirmed by restriction mapping and hybridization to specificoligonucleotides, while that of the GPIX cosmid was verified by direct sequencing of cosmid DNA. Sequencing reactions were performed using the