Ceratocystis larium sp. nov., a new species from Styrax benzoin wounds associated with incense harvesting in Indonesia.
Ceratocystis larium sp. nov., a new species from Styrax benzoin wounds associated with incense harvesting in Indonesia.
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DOI：
10.3767/003158509x439076
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发表时间：
2009-06
期刊：
影响因子：
9.1
通讯作者：
Wingfield MJ
中科院分区：
文献类型：
--
作者：
van Wyk M;Wingfield BD;Clegg PA;Wingfield MJ
安息香树，原产于苏门答腊岛，印度尼西亚受伤产生树脂，收集和燃烧作为香。树木上的这些伤口通常会发展成不断扩大的溃疡，导致树木死亡。这项研究的目的是考虑是否Ophiostomatoid真菌，通常与树木的伤口可能与树脂收获S。安息香从人工诱导伤口的边缘收集样本，特别是在溃疡和血管组织染色明显的地方。将组织样品在潮湿室中孵育，并使用胡萝卜诱饵来检测长喙壳属的存在。结果结构具有典型的C.菌毛公司在培养的木材和胡萝卜诱饵上均发现了种复合体和无性型属Thielaviopsis的种。生成内转录间隔区1和2的DNA序列，包括5.8S rRNA基因、部分β-微管蛋白和转录延伸因子1-α基因区域。这些数据与C.菌毛公司种复合体和梭孢霉属的系统发育分析。形态学观察和系统发育分析表明，伤口上的拟梭孢霉属为拟梭孢霉。基生菌。分离到的长喙壳属（Ceratocystis sp.）安息香是C.菌毛公司这里描述为C。落叶松属新种
Styrax benzoin trees, native to the island Sumatra, Indonesia are wounded to produce resin that is collected and burned as incense. These wounds on trees commonly develop into expanding cankers that lead to tree death. The aim of this study was to consider whether Ophiostomatoid fungi, typically associated with wounds on trees might be associated with resin harvesting on S. benzoin. Samples were collected from the edges of artificially induced wounds, and particularly where cankers and staining of the vascular tissue was evident. Tissue samples were incubated in moist chambers and carrot baiting was also used to detect the presence of Ceratocystis spp. Fruiting structures with morphology typical of species in the C. fimbriata s.l. species complex and species in the anamorph genus Thielaviopsis were found, on both the incubated wood and the carrot baits. DNA sequences were generated for the Internal Transcribed Spacer regions 1 and 2 including the 5.8S rRNA gene, part of the β-tubulin and the Transcription Elongation Factor 1-α gene regions. These data were compared with those of other species in the C. fimbriata s.l. species complex and Thielaviopsis using phylogenetic analysis. Morphology of the isolates in culture as well as phylogenetic inference showed that the Thielaviopsis sp. present on the wounds was T. basicola. The Ceratocystis sp. from S. benzoin represents a new taxon in the C. fimbriata s.l. complex described here as C. larium sp. nov.